@article{fdi:010090607, title = {{D}etection of {H}epatitis {E} {V}irus ({HEV}) in pork sold in {S}aint-{L}ouis, the {N}orth of {S}enegal}, author = {{T}ene, {S}. {D}. and {D}iouara, {A}. {M}. and {K}ane, {A}. and {S}an{\'e}, {S}. and {C}oundoul, {S}. and {T}hiam, {F}. and {N}guer, {C}. {M}. and {D}iop, {M}. and {M}baye, {M}. {N}. and {M}bengue, {M}. and {L}o, {S}. {Y}. {B}. and {N}diaye, {H}. {D}. and {K}ane, {C}. {T}. and {A}youba, {A}hidjo}, editor = {}, language = {{ENG}}, abstract = {{T}he hepatitis {E} virus ({HEV}) is a zoonotic pathogen with various hosts, including pigs, which act as reservoirs. {I}n industrialized countries, sporadic cases caused by genotype 3, contracted by ingesting contaminated uncooked or undercooked meat, have been reported. {H}owever, in developing countries, {HEV} infection is mainly dominated by genotype 2 and often associated with poor hygiene conditions and drinking water supplies. {HEV} infection and its circulation in domestic fauna in {W}est {A}frica are poorly documented. {T}his study aimed to assess the presence of {HEV} in pork sold in {S}aint-{L}ouis, {S}enegal. {M}eat products (250 g samples, n = 74) were purchased in {A}ugust 2022 from three locations. {T}hen, 2 g/sample was minced to extract total nucleic acids using the {P}urelink ({TM}) {V}iral {DNA}/{RNA} kit. {RT}-{PCR} reactions were performed using the {O}ne-{T}aq ({TM}) {O}ne-{S}tep {RT}-{PCR} kit targeting the {HEV} {ORF}2 genomic region. {T}he products obtained were visualized on a 1% agarose gel. {O}f a total of 74 samples, divided into pork meat (n = 65) and pork liver (n = 9), 5.4% (n = 4) tested positive for {HEV}. {I}n both cases, two samples were positive, representing a rate of 3.1% and 22.2% for meat and pork liver, respectively. {A}ll new viral sequences were obtained from a monophyletic group within {HEV} genotype 3. {T}his study is the first to report the presence of {HEV} in pork sold in {S}enegal and the results reveal a potential circulation of {HEV} in the pig population. {T}he high proportion of contamination in the pork liver samples highlights a major risk associated with their consumption.}, keywords = {hepatitis {E} virus ; {HEV}-3 ; foodborne disease ; domestic wildlife ; zoonotic ; risk ; {S}aint-{L}ouis ; {SENEGAL}}, booktitle = {}, journal = {{L}ife}, volume = {14}, numero = {4}, pages = {512 [12 p.]}, year = {2024}, DOI = {10.3390/life14040512}, URL = {https://www.documentation.ird.fr/hor/fdi:010090607}, }