@article{fdi:010090589,
  title = {{R}ice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in {N}icotiana benthamiana leaves},
  author = {{B}amogo, {P}. {K}. {A}. and {T}iendr{\'e}b{\'e}ogo, {F}. and {B}rugidou, {C}hristophe and {S}{\'e}r{\'e}m{\'e}, {D}. and {D}jigma, {F}. and {S}impor{\'e}, {J}. and {L}acombe, {S}{\'e}verine},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {S}ince the 2000's, plants have been used as bioreactors for the transient production of molecules of interest such as vaccines. {T}o improve protein yield, "amplicon" vectors based on plant viruses are used. {T}hese viral constructs, engineered to carry the gene of interest replicate strongly once introduced into the plant cell, allowing significant accumulation of the protein. {H}ere, we evaluated the suitability of the monocot-infecting {RNA} virus {R}ice yellow mottle virus ({RYMV}) as an amplicon vector. {T}he promastigote surface antigen ({PSA}) of the protozoan {L}eishmania was considered as a protein of interest due to its vaccine properties against canine leishmaniasis. {R}esults {S}ince {P}1 ({ORF}1) and {CP} ({ORF}3) proteins are not strictly necessary for viral replication, {ORF}1 was deleted and the {PSA} gene was substituted to {ORF}3 in the {RYMV}-based vector. {W}e evaluated its expression in the best described plant bioreactor system, {N}icotiana benthamiana which, unlike rice, allows transient transformation by {A}grobacterium. {D}espite not being its natural host, we demonstrated a low level of {RYMV}-based vector replication in {N}. benthamiana leaves. {U}nder optimized ratio, we showed that the {P}19 silencing suppressor in combination with the missing viral {CP} {ORF} significantly enhanced {RYMV} amplicon replication in {N}. benthamiana. {U}nder these optimized {CP}/{P}19 conditions, we showed that the {RYMV} amplicon replicated autonomously in the infiltrated {N}. benthamiana cells, but was unable to move out of the infiltrated zones. {F}inally, we showed that when the {RYMV} amplicon was expressed under the optimized conditions we set up, it allowed enhanced {PSA} protein accumulation in {N}. benthamiana compared to the {PSA} coding sequence driven by the 35{S} promoter without amplicon background. {C}onclusion {T}his work demonstrates that a non-dicot-infecting virus can be used as an amplicon vector for the efficient production of proteins of interest such as {PSA} in {N}. benthamiana leaves.},
  keywords = {{PSA} ; {RYMV} ; {P}lant-based viral vector ; {L}eishmaniosis},
  booktitle = {},
  journal = {{BMC} {B}iotechnology},
  volume = {24},
  numero = {1},
  pages = {21 [12 p.]},
  year = {2024},
  DOI = {10.1186/s12896-024-00851-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090589},
}