@article{fdi:010090536,
  title = {{G}enetic diversity of endosymbiotic bacteria {W}olbachia infecting two mosquito species of the genus {E}retmapodites occurring in sympatry in the {C}omoros archipelago},
  author = {{G}omard, {Y}. and {H}afsia, {S}. and {L}ebon, {C}yrille and {R}abarison, {P}. and {I}daroussi, {A}. {B}. and {Y}ssouf, {A}. and {B}ouss{\`e}s, {P}hilippe and {M}avingui, {P}. and {A}tyame, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}ntroduction: {T}he influence of {W}olbachia on mosquito reproduction and vector competence has led to renewed interest in studying the genetic diversity of these bacteria and the phenotypes they induced in mosquito vectors. {I}n this study, we focused on two species of {E}retmapodites, namely {E}retmapodites quinquevittatus and {E}retmapodites subsimplicipes, from three islands in the {C}omoros archipelago (in the {S}outhwestern {I}ndian {O}cean). {M}ethods: {U}sing the {COI} gene, we examined the mitochondrial genetic diversity of 879 {E}retmapodites individuals from 54 sites. {A}dditionally, we investigated the presence and genetic diversity of {W}olbachia using the wsp marker and the diversity of five housekeeping genes commonly used for genotyping through {M}ultiple {L}ocus {S}equence {T}yping ({MLST}). {R}esults and discussion: {O}verall, {E}r. quinquevittatus was the most abundant species in the three surveyed islands and both mosquito species occurred in sympatry in most of the investigated sites. {W}e detected a higher mitochondrial genetic diversity in {E}r. quinquevittatus with 35 reported haplotypes ({N} = 615 specimens, {H}d = 0.481 and pi = 0.002) while 13 haplotypes were found in {E}r. subsimplicipes ({N} = 205 specimens, {H}d = 0.338 and pi = 0.001), this difference is likely due to the bias in sampling size between the two species. {W}e report for the first time the presence of {W}olbachia in these two {E}retmapodites species. {T}he prevalence of {W}olbachia infection varied significantly between species, with a low prevalence recorded in {E}r. quinquevittatus (0.8%, {N} = 5/627) while infection was close to fixation in {E}r. subsimplicipes (87.7%, {N} = 221/252). {B}oth male and female individuals of the two mosquito species appeared to be infected. {T}he analysis of {MLST} genes revealed the presence of two {W}olbachia strains corresponding to two new strain types ({ST}s) within the supergroups {A} and {B}, which have been named w{E}ret{A} and w{E}ret{B}. {T}hese strains were found as mono-infections and are closely related, phylogenetically, to {W}olbachia strains previously reported in {D}rosophila species. {F}inally, we demonstrate that maternal transmission of {W}olbachia is imperfect in {E}r. subsimplicipes, which could explain the presence of a minority of uninfected individuals in the field.},
  keywords = {{W}olbachia ; {E}retmapodites quinquevittatus ; {E}retmapodites subsimplicipes ; mitochondrial genetic diversity ; {C}omoros archipelago ; {COMORES}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {15},
  numero = {},
  pages = {1343917 [15 p.]},
  year = {2024},
  DOI = {10.3389/fmicb.2024.1343917},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090536},
}