@article{fdi:010090329,
  title = {{G}enomic epidemiology of carbapenemase-producing {G}ram-negative bacteria at the human-animal-environment interface in {D}jibouti city, {D}jibouti},
  author = {{M}ohamed, {H}. {S}. and {G}alal, {L}. and {H}ayer, {J}uliette and {B}enavides, {J}. {A}. and {B}anuls, {A}nne-{L}aure and {D}upont, {C}. and {C}onquet, {G}. and {C}arri{\`e}re, {C}. and {D}umont, {Y}. and {D}idelot, {M}. {N}. and {M}ichon, {A}. {L}. and {J}ean-{P}ierre, {H}. and {A}boubaker, {M}. {H}. and {G}odreuil, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he emergence of carbapenem resistance is a major public health threat in sub-{S}aharan {A}frica but remains poorly understood, particularly at the human-animal-environment interface. {T}his study provides the first {O}ne {H}ealth based study on the epidemiology of {C}arbapenemase-{P}roducing {G}ram-{N}egative {B}acteria ({CP}-{GNB}) in {D}jibouti {C}ity, {D}jibouti, {E}ast {A}frica. {I}n total, 800 community urine samples and 500 hospital specimens from humans, 270 livestock fecal samples, 60 fish samples, and 20 water samples were collected and tested for carbapenem resistance. {T}he overall estimated {CP}-{GNB} prevalence was 1.9 % (32/1650 samples) and specifically concerned 0.3 % of community urine samples, 2.8 % of clinical specimens, 2.6 % of livestock fecal samples, 11.7 % of fish samples, and 10 % of water samples. {T}he 32 {CP}-{GNB} included 19 {E}scherichia coli, seven {A}cinetobacter baumannii, five {K}lebsiella pneumoniae, and one {P}roteus mirabilis isolate. {S}hort-read ({I}llumina) and long-read ({N}anopore) genome sequencing revealed that carbapenem resistance was mainly associated with chromosomal carriage of bla{NDM}-1, bla{OXA}-23, bla{OXA}-48, bla{OXA}-66, and bla{OXA}-69 in {A}. baumannii, and with plasmid carriage in {E}nterobacterales (bla{NDM}-1 and bla{OXA}-181 in {E}. coli, bla{NDM}-1, bla{NDM}-5 and bla{OXA}-48 in {K}. pneumoniae, and bla{NDM}-1 in {P}. mirabilis). {M}oreover, 17/32 {CP}-{GNB} isolates belonged to three epidemic clones: (1) {A}. baumannii sequence type ({ST}) 1697,2535 that showed a distribution pattern consistent with intra-and inter-hospital dissemination; (2) {E}. coli {ST}10 that circulated at the human-animal-environment interface; and (3) {K}. pneumoniae {ST}147 that circulated at the human-environment interface. {H}orizontal exchanges probably contributed to carbapenem resistance dissemination in the city, especially the bla{OXA}-181-carrying {C}ol{KP}3-{I}nc{X}3 hybrid plasmid that was found in {E}. coli isolates belonging to different {ST}s. {O}ur study highlights that despite a relatively low {CP}-{GNB} prevalence in {D}jibouti {C}ity, plasmids harboring carbapenem resistance circulate in humans, animals and environment. {O}ur findings stress the need to implement preventive and control measures for reducing the circulation of this potentially emerging public health threat.},
  keywords = {{E}mergence ; {A}ntibiotic resistance ; {C}arbapenemase ; {O}ne {H}ealth ; {A}frica ; {DJIBOUTI}},
  booktitle = {},
  journal = {{S}cience of the {T}otal {E}nvironment},
  volume = {905},
  numero = {},
  pages = {167160 [10 ]},
  ISSN = {0048-9697},
  year = {2023},
  DOI = {10.1016/j.scitotenv.2023.167160},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090329},
}