@article{fdi:010090289,
  title = {{C}ryopreservation of indigenous plums and monitoring of multiplication and rooting capacity of shoots obtained from cryopreserved specimens},
  author = {{V}ujovic, {T}. and {A}ndelic, {T}. and {V}asilijevic, {B}. and {J}evremovic, {D}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he objective of this study is to assess the suitability of vitrification cryo-plate ({V} cryoplate) and dehydration cryo-plate ({D} cryo-plate) methods for the long-term conservation of eight autochthonous {P}runus domestica {L}. genotypes originating from the {B}alkan {P}eninsula region. {I}n vitro shoot tips were briefly pre-cultured for 1 day at 23 degrees {C} in the dark on a medium containing 0.3 {M} sucrose and then embedded in calcium alginate gel within the wells of the aluminum cryo-plates. {I}n the {V} cryo-plate protocol, dehydration was carried out at room temperature using the following vitrification solutions: original plant vitrification solution 2 ({PVS}2) and 90% {PVS}2 solution (for 20 and 40 min) and plant vitrification solution 3 ({PVS}3) (for 60 and 80 min). {I}n the {D} cryo-plate protocol, desiccation was performed for 2, 2.5, or 3 h over silica gel at 23 degrees {C}. {T}he effect of different treatments was evaluated by monitoring the regrowth of both non-frozen and cryo-preserved explants. {A}fter cryo-preservation, five genotypes achieved regrowth rates over 40% in at least one of the applied protocols, while two genotypes showed regrowth rates of around 10%. {A} significant improvement in regrowth success for all genotypes using both cryo-plate methods was achieved by pre-culturing shoot tips for 7 days on a medium containing 0.5{M}sucrose in complete darkness at 4 degrees {C}. {S}hoots regenerated from cryo-preserved explants were further monitored in vitro. {B}y the third subculture, they had not only regained but had even exceeded the multiplication capacity (index of multiplication, length of axial, and lateral shoots) of shoots regenerated from dissection controls. {F}ollowing multiplication, the cryo-preserved shoots were successfully rooted and rooting ability was assessed by monitoring the percentage of rooting, number and length of roots, and height of rooted plantlets.},
  keywords = {{P}runus domestica {L}. ; plant vitrification solution ; {V} cryo-plate ; desiccation ; {D} cryo-plate ; regrowth ; multiplication ; rooting},
  booktitle = {},
  journal = {{P}lants},
  volume = {12},
  numero = {17},
  pages = {3108 [21 p.]},
  year = {2023},
  DOI = {10.3390/plants12173108},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090289},
}