@article{fdi:010090194,
  title = {{A}ssociation between polymorphisms of {IL}4, {IL}13, {IL}10, {STAT}6 and {IFNG} genes, cytokines and immunoglobulin {E} levels with high burden of {S}chistosoma mansoni in children from schistosomiasis endemic areas of {C}ameroon},
  author = {{M}ewamba, {E}. {M}. and {N}oyes, {H}. and {T}iofack, {A}. {A}. {Z}. and {K}amga, {R}. {M}. {N}. and {K}amdem, {C}. {N}. and {M}engoue, {L}. {E}. {T}. and {O}fon, {E}. and {N}gassam, {R}. {I}. {K}. and {N}yangiri, {O}. and {B}ucheton, {B}runo and {N}jiokou, {F}. and {W}omeni, {M}. {H}. and {M}atovu, {E}. and {M}ac{L}eod, {A}. and {S}imo, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}liminating schistosomiasis as a public health problem by 2030 requires a better understanding of the disease transmission, especially the asymmetric distribution of worm burden in individuals living and sharing the same environment. {I}t is in this light that this study was designed to identify human genetic determinants associated with high burden of {S}. mansoni and also with the plasma concentrations of {I}g{E} and four cytokines in children from two schistosomiasis endemic areas of {C}ameroon. {I}n school-aged children of schistosomiasis endemic areas of {M}akenene and {N}om-{K}andi of {C}ameroon, {S}. mansoni infections and their infection intensities were evaluated in urine and stool samples using respectively the {P}oint-of-care {C}irculating {C}athodic {A}ntigen test ({POC}-{CCA}) and the {K}ato {K}atz ({KK}) test. {T}hereafter, blood samples were collected in children harbouring high burden of schistosome infections as well as in their parents and siblings. {DNA} extracts and plasma were obtained from blood. {P}olymorphisms at 14 loci of five genes were assessed using {PCR}-restriction fragment length polymorphism and amplification-refractory mutation system. {T}he {ELISA} test enabled to determine the plasma concentrations of {I}g{E}, {IL}-13, {IL}-10, {IL}-4 and {IFN}-?. {T}he prevalence of {S}. mansoni infections was significantly higher ({P} < 0.0001 for {POC}-{CCA}; {P} = 0.001 for {KK}) in {M}akenene (48.6% for {POC}-{CCA} and 7.9% for {KK}) compared to {N}om-{K}andi (31% for {POC}-{CCA} and 4.3% for {KK}). {T}he infection intensities were also higher ({P} < 0.0001 for {POC}-{CCA}; {P} = 0.001 for {KK}) in children from {M}akenene than those from {N}om-{K}andi. {T}he allele {C} of {SNP} rs3024974 of {STAT}6 was associated with an increased risk of bearing high burden of {S}. mansoni both in the additive (p = 0.009) and recessive model (p = 0.01) while the allele {C} of {SNP} rs1800871 of {IL}10 was protective (p = 0.0009) against high burden of {S}. mansoni. {T}he alleles {A} of {SNP} rs2069739 of {IL}13 and {G} of {SNP} rs2243283 of {IL}4 were associated with an increased risk of having low plasma concentrations of {IL}-13 ({P} = 0.04) and {IL}-10 ({P} = 0.04), respectively. {T}his study showed that host genetic polymorphisms may influence the outcome (high or low worm burden) of {S}. mansoni infections and also the plasma concentrations of some cytokines.},
  keywords = {{S}chistosoma mansoni ; {C}irculating cathodic antigen ; {C}ytokines ; {I}g{E} ; {G}enetic polymorphism ; {I}nfection intensity ; {CAMEROUN}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {111},
  numero = {},
  pages = {105416 [12 p.]},
  ISSN = {1567-1348},
  year = {2023},
  DOI = {10.1016/j.meegid.2023.105416},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090194},
}