Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Pathogens 974 [11 ] enterobacteria carbapenemase colistin SENEGAL 2023 fdi:010090174 ENG Pathogens ISI:001055278900001 8 10.3390/pathogens12080974 https://www.documentation.ird.fr/hor/fdi:010090174 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-10/010090174.pdf 12 Horizon (IRD) Antibiotic resistance is a public health problem. The emergence of carbapenemase-producing Enterobacterales (CPE) infections is a concern, particularly in Senegal. (1) Methods: Between January 2019 and July 2022, 240 isolates of enterobacteria resistant to third-generation cephalosporins and imipenem from biological samples from Fann Hospital (Dakar) and Hopital Paix (Ziguinchor) were selected. The isolates were identified by MALDI-TOF mass spectrometry, and susceptibility tests were performed by the disk diffusion method. Antibiotic-resistance genes for class A beta-lactamases, carbapenemases, and plasmid resistance to colistin resistance (mcr-1-8) were screened by RT-PCR. (2) Results: The 240 enterobacteria were composed of: Escherichia coli (60.83%), Klebsiella pneumoniae (21.67%), Enterobacter cloacae (13.75%), Citrobacter freundii (2.08%), Serratia marcescens (0.83%), Klebsiella aerogenes (0.42%), and Proteus mirabilis (0.42%). Class A beta-lactamase genes were found in 229 isolates (70.41% bla(TEM), 37.5% bla(SHV), 83.75% bla(CTX-A), and 0.42% bla(CTX-B)). The carbapenemase genes bla(OXA-48) and bla(NDM) were found in 25 isolates, including 14 isolates with bla(OXA-48), 13 isolates with bla(NDM), and 2 isolates with both genes simultaneously. The mcr-8 gene was found in one isolate of E. cloacae. (3) Conclusions: The epidemiology of antibiotic-resistance genes in enterobacteria in Senegal shows the emergence of CPEs. This phenomenon is worrying, and rigorous surveillance is necessary to avoid further spread. 084 ; 050 ; 020 UR258 / UR257 Sénégal