%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Larreche, S.
%A Bousquet, A.
%A da Silva, L.
%A Planelles, A.
%A Ksas, R.
%A Merens, A.
%A Chippaux, Jean-Philippe
%T Antibiotic susceptibility of cultivable microbiota from the oral cavity of captive Bothrops atrox and Bothrops lanceolatus : implications for the treatment of snakebite-associated infections in the French departments of America
%D 2023
%L fdi:010090164
%G ENG
%J Infectious Diseases Now
%@ 2666-9927
%K Snakebite ; Antibiotic resistance ; French Guiana ; Martinique
%K GUYANE FRANCAISE ; ANTILLES
%M ISI:001055675400001
%N 7
%P 104721 [5 ]
%R 10.1016/j.idnow.2023.104721
%U https://www.documentation.ird.fr/hor/fdi:010090164
%> https://www.documentation.ird.fr/intranet/publi/2023-10/010090164.pdf
%V 53
%W Horizon (IRD)
%X Introduction: Secondary infection is a frequent complication after bites by Bothrops atroxin French Guiana or B. lanceolatus in Martinique. Knowledge of the bacteria present in snake mouths is a valuable aid for determining probabilistic antibiotherapy after Bothrops bite. The objectives of this study were to describe the cultivable bacteria of the oral microbiota of the specimens of B. atrox and B. lanceolatus kept in captivity, and to study their susceptibility to antibiotics.Methods: Fifteen B. atrox and 15 B. lanceolatus were sampled. Bacterial cultures were performed and each morphotype on plates was identified using MALDI-TOF mass spectrometry. Antibiotic susceptibility was studied using the agar disk diffusion method, with possible determination of the MICs.Results: One hundred and twenty-two isolates were identified: 52 isolates and 13 species in B. atrox, 70 isolates and 23 species in B. lanceolatus. The main species were Providencia rettgeri, Morganella morganii, Pseudomonas aeruginosa, Staphylococcus xylosus, and Paeniclostridium sordellii (only in B. lanceolatus mouths). For B. atrox, 96% of isolates were susceptible to piperacillin/tazobactam, cefepime, imipenem and meropenem, 94% to ciprofloxacin and 76% to cefotaxime and ceftriaxone. For B. lanceolatus, 97% of isolates were susceptible to meropenem, 96% to cefepime, 93% to imipenem and piperacillin/tazobactam, 80% to ciprofloxacin, and 75% to cefotaxime and ceftriaxone. Many isolates were resistant towards amoxicillin/clavulanate. Conclusion: Among currently recommended antibiotics, cefepime and piperacillin/tazobactam seem more suitable than cefotaxime or ceftriaxone in the event of a Bothrops bite. Ciprofloxacin may also be considered for B. atrox.
%$ 050 ; 080