@article{fdi:010090142,
  title = {p{WCP} is a widely distributed and highly conserved {W}olbachia plasmid in {C}ulex pipiens and {C}ulex quinquefasciatus mosquitoes worldwide},
  author = {{G}housein, {A}. and {T}utagata, {J}. and {S}chrieke, {H}. and {E}tienne, {M}. and {C}haumeau, {V}. and {B}oyer, {S}. and {P}ages, {N}. and {R}oiz, {D}avid and {E}ren, {A}. {M}. and {C}ambray, {G}. and {R}eveillaud, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}osquitoes represent the most important pathogen vectors and are responsible for the spread of a wide variety of poorly treatable diseases. {W}olbachia are obligate intracellular bacteria that are widely distributed among arthropods and collectively represents one of the most promising solutions for vector control. {I}n particular, {W}olbachia has been shown to limit the transmission of pathogens, and to dramatically affect the reproductive behavior of their host through its phage {WO}. {W}hile much research has focused on deciphering and exploring the biocontrol applications of these {WO}-related phenotypes, the extent and potential impact of the {W}olbachia mobilome remain poorly appreciated. {N}otably, several {W}olbachia plasmids, carrying {WO}-like genes and {I}nsertion {S}equences ({IS}), thus possibly interrelated to other genetic units of the endosymbiont, have been recently discovered. {H}ere we investigated the diversity and biogeography of the first described plasmid of {W}olbachia in {C}ulex pipiens (p{WCP}) in several islands and continental countries around the world-including {C}ambodia, {G}uadeloupe, {M}artinique, {T}hailand, and {M}exico-together with mosquito strains from colonies that evolved for 2 to 30 years in the laboratory. {W}e used {PCR} and q{PCR} to determine the presence and copy number of p{WCP} in individual mosquitoes, and highly accurate {S}anger sequencing to evaluate potential variations. {T}ogether with earlier observation, our results show that p{WCP} is omnipresent and strikingly conserved among {W}olbachia populations within mosquitoes from distant geographies and environmental conditions. {T}hese data suggest a critical role for the plasmid in {W}olbachia ecology and evolution, and the potential of a great tool for further genetic dissection and possible manipulation of this endosymbiont.},
  keywords = {{CAMBODGE} ; {THAILANDE} ; {GUADELOUPE} ; {MARTINIQUE} ; {MEXIQUE}},
  booktitle = {},
  journal = {{ISME} {C}ommunications},
  volume = {3},
  numero = {1},
  pages = {40 [5 p.]},
  year = {2023},
  DOI = {10.1038/s43705-023-00248-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090142},
}