@article{fdi:010090068,
  title = {{M}olecular identification of enteric viruses in domestic animals in {N}ortheastern {G}abon, {C}entral {A}frica},
  author = {{K}ombila, {L}. {B}. and {N}'{D}ilimabaka, {N}. and {G}arcia, {D}. and {R}ieu, {O}. and {O}ndo, {J}. {D}. {E}. and {M}ebaley, {T}. {N}. and {B}oundenga, {L}. and {F}ritz, {M}atthieu and {L}enguiya, {L}. {H}. and {M}aganga, {G}. {D}. and {L}eroy, {E}ric and {B}ecquart, {P}ierre and {M}ombo, {I}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}imple {S}ummary {E}nteric viruses cause gastroenteritis in humans and animals, but have also been associated with several extraintestinal diseases. {T}hese viruses affect a wide range of vertebrate species, including birds and mammals. {F}requent and close contact between humans and animals can potentially lead to the emergence of zoonoses. {A}pproximately 60% of zoonotic diseases in humans are spillovers from wildlife. {P}reventing future outbreaks of emerging zoonotic diseases calls for better description of the viruses that circulate in domestic animals, because they are located at the human/wildlife interface. {W}e therefore screened for the presence of astroviruses, enteroviruses, and caliciviruses-three of the main viral families causing enteric diseases both in humans and animals-in goats, sheep and dogs living in villages in northeastern {G}abon. {W}e identified the presence of astroviruses in goats, a calicivirus in a dog, and enteroviruses in all three species. {A}ll detected viruses were animal-related, but not those from wildlife. {H}owever, we showed that some human-pathogenic enteroviruses infect goats and dogs. {T}herefore, further studies are required to better understand the role of domestic animals as amplifiers of reverse zoonotic viruses. {A}stroviruses ({A}st{V}s), enteroviruses ({EV}s), and caliciviruses ({C}a{V}s) infect several vertebrate taxa. {T}ransmitted through the fecal-oral route, these enteric viruses are highly resistant and can survive in the environment, thereby increasing their zoonotic potential. {H}ere, we screened for {A}st{V}s, {EV}s, and {C}a{V}s to investigate the role of domestic animals in the emergence of zoonoses, because they are situated at the human/wildlife interface, particularly in rural forested areas in {C}entral {A}frica. {R}ectal swabs were obtained from 123 goats, 41 sheep, and 76 dogs in 10 villages located in northeastern {G}abon. {E}xtracted {RNA} reverse-transcribed into c{DNA} was used to detect {A}st{V}s, {EV}s, and {C}a{V}s by amplification of the {RNA}-dependent {RNA} polymerase ({R}d{R}p), or capsid protein ({VP}1) gene using {PCR}. {A} total of 23 samples tested positive, including 17 goats for {A}st{V}s, 2 goats, 2 sheep, 1 dog for {EV}s, and 1 dog for {C}a{V}s. {P}hylogenetic analyses revealed that {A}st{V} {R}d{R}p sequences clustered with sheep-, goat-, or bovine-related {A}st{V}s. {I}n addition, one goat and two sheep {VP}1 sequences clustered with caprine/ovine-related {E}vs within the {E}nterovirus {G} species, and the {C}a{V} was a canine vesivirus. {H}owever, human-pathogenic {E}vs, {EV}-{B}80 and {EV}-{C}99, were detected in goats and dogs, raising questions on the maintenance of viruses able to infect humans.},
  keywords = {astroviruses ; enteroviruses ; caliciviruses ; vesivirus ; small ruminants ; dogs ; zoonoses ; rural areas ; {GABON}},
  booktitle = {},
  journal = {{A}nimals},
  volume = {13},
  numero = {15},
  pages = {2512 [13 p.]},
  ISSN = {2076-2615},
  year = {2023},
  DOI = {10.3390/ani13152512},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090068},
}