@article{fdi:010090051,
  title = {{TRIMETHYLGUANOSINE} {SYNTHASE}1 mutations decanalize female germline development in {A}rabidopsis},
  author = {{S}iena, {L}. {A}. and {M}ichaud, {C}aroline and {S}elles, {B}enjamin and {V}ega, {J}. {M}. and {P}essino, {S}. {C}. and {I}ngouff, {M}. and {O}rtiz, {J}. {P}. {A}. and {L}eblanc, {O}livier},
  editor = {},
  language = {{ENG}},
  abstract = {{H}ere, we report the characterization of a plant {RNA} methyltransferase, orthologous to yeast trimethylguanosine synthase1 ({T}gs1p) and whose downregulation was associated with apomixis in {P}aspalum grasses.{U}sing phylogenetic analyses and yeast complementation, we determined that land plant genomes all encode a conserved, specific {TGS}1 protein. {N}ext, we studied the role of {TGS}1 in female reproduction using reporter lines and loss-of-function mutants in {A}rabidopsis thaliana.p{A}t{TGS}1:{A}t{TGS}1 reporters showed a dynamic expression pattern. {T}hey were highly active in the placenta and ovule primordia at emergence but, subsequently, showed weak signals in the nucellus. {A}lthough expressed throughout gametophyte development, activity became restricted to the female gamete and was also detected after fertilization during embryogenesis. {TGS}1 depletion altered the specification of the precursor cells that give rise to the female gametophytic generation and to the sporophyte, resulting in the formation of a functional aposporous-like lineage.{O}ur results indicate that {TGS}1 participates in the mechanisms restricting cell fate acquisition to a single cell at critical transitions throughout the female reproductive lineage and, thus, expand our current knowledge of the mechanisms governing female reproductive fate in plants.},
  keywords = {apospory ; {A}rabidopsis ; female reproductive lineage ; {RNA} ; methyltransferase ; {TGS}1},
  booktitle = {},
  journal = {{N}ew {P}hytologist},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[16 p.]},
  ISSN = {0028-646{X}},
  year = {2023},
  DOI = {10.1111/nph.19179},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090051},
}