Grégoire P. auteur aut IRD Bohli M. auteur aut IRD Cayol J.L. auteur aut IRD Joseph M. auteur aut IRD Guasco S. auteur aut IRD Dubourg K. auteur aut IRD Cambar J. auteur aut IRD Michotey V. auteur aut IRD Bonin P. auteur aut IRD Fardeau Marie-Laure auteur aut IRD Ollivier Bernard auteur aut IRD text journalArticle eng text/pdf reformatted digital access An anaerobic, thermophilic, filamentous (0.45 x >100 mu m) bacterium, designated D1-25-10-4(T), was isolated from a deep hot aquifer in France. Cells were non-motile and Gram-negative. Growth was observed at 43-65 degrees C (optimum 55 degrees C), at pH 6.8-7.8 (optimum pH 7.0) and with 0-5 g NaCl l(-1) (optimum 0 g NaCl l(-1)). Strain D1-25-10-4(T). was a chemo-organotroph and fermented ribose, maltose, glucose, galactose, arabinose, fructose, mannose, sucrose, raffinose, xylose, glycerol, fumarate, peptone, starch and xylan. Yeast extract was required for growth. Sulfate, thiosulfate, sulfite, elemental sulfur, nitrate, nitrite and fumarate were not used as terminal electron acceptors. The G+C content of the DNA was 61.9 mol%. The major cellular fatty acids of strain D1-25-10-4(T) were C-17:0, C-18:0, C-16:0 and iso-C-17:0. The closest phylogenetic relative of strain D1-25-10-4(T) was Caldilinea aerophila STL-6-01(T) (97.9 % 16S rRNA gene sequence similarity). DNA-DNA relatedness between strain D1-25-10-4(T) and Caldilinea aerophila DSM 14535(T) was 8.7 +/- 1 %. On the basis of phylogenetic, genotypic and phenotypic characteristics, strain D1-25-10-4(T) represents a novel species within the genus Caldilinea, class Caldilineae, phylum Chloroflexi, for which the name Caldilinea tarbellica sp. nov. is proposed. The type strain is D1-25-10-4(T) (=DSM 22659(T) =JCM 16120(T)). specialized 034 084 61 Part 6 1436-1441 2011 1466-5026 https://www.documentation.ird.fr/hor/fdi:010089997 10.1099/ijs.0.025676-0 1466-5026 [F B010089997] https://www.documentation.ird.fr/hor/fdi:010089997 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-07/010089997.pdf IRD - Base Horizon / Pleins textes 2011-08-03 2024-07-03 fdi:010089997 fre