Voichek Y. auteur aut IRD Hurieva B. auteur aut IRD Michaud Caroline auteur aut IRD Schmücker A. auteur aut IRD Vergara Z. auteur aut IRD Desvoyes B. auteur aut IRD Gutierrez C. auteur aut IRD Nizhynska V. auteur aut IRD Jaegle B. auteur aut IRD Borg M. auteur aut IRD Berger F. auteur aut IRD Nordborg M. auteur aut IRD Ingouff M. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Fertilization in Arabidopsis (Arabidopsis thaliana) is a highly coordinated process that begins with a pollen tube delivering the 2 sperm cells into the embryo sac. Each sperm cell can then fertilize either the egg or the central cell to initiate embryo or endosperm development, respectively. The success of this double fertilization process requires a tight cell cycle synchrony between the male and female gametes to allow karyogamy (nuclei fusion). However, the cell cycle status of the male and female gametes during fertilization remains elusive as DNA quantification and DNA replication assays have given conflicting results. Here, to reconcile these results, we quantified the DNA replication state by DNA sequencing and performed microscopic analyses of fluorescent markers covering all phases of the cell cycle. We show that male and female Arabidopsis gametes are both arrested prior to DNA replication at maturity and initiate their DNA replication only during fertilization. Mature male and female Arabidopsis gametes arrest their cell cycle before DNA replication, which only starts during fertilization. specialized 076 [Early access] [10 p.] 2023 0032-0889 https://www.documentation.ird.fr/hor/fdi:010089654 10.1093/plphys/kiad512 0032-0889 [F B010089654] https://www.documentation.ird.fr/hor/fdi:010089654 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-04/010089654.pdf IRD - Base Horizon / Pleins textes 2024-04-05 2024-04-05 fdi:010089654 fre