@article{fdi:010089654,
  title = {{C}ell cycle status of male and female gametes during {A}rabidopsis reproduction},
  author = {{V}oichek, {Y}. and {H}urieva, {B}. and {M}ichaud, {C}aroline and {S}chmücker, {A}. and {V}ergara, {Z}. and {D}esvoyes, {B}. and {G}utierrez, {C}. and {N}izhynska, {V}. and {J}aegle, {B}. and {B}org, {M}. and {B}erger, {F}. and {N}ordborg, {M}. and {I}ngouff, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{F}ertilization in {A}rabidopsis ({A}rabidopsis thaliana) is a highly coordinated process that begins with a pollen tube delivering the 2 sperm cells into the embryo sac. {E}ach sperm cell can then fertilize either the egg or the central cell to initiate embryo or endosperm development, respectively. {T}he success of this double fertilization process requires a tight cell cycle synchrony between the male and female gametes to allow karyogamy (nuclei fusion). {H}owever, the cell cycle status of the male and female gametes during fertilization remains elusive as {DNA} quantification and {DNA} replication assays have given conflicting results. {H}ere, to reconcile these results, we quantified the {DNA} replication state by {DNA} sequencing and performed microscopic analyses of fluorescent markers covering all phases of the cell cycle. {W}e show that male and female {A}rabidopsis gametes are both arrested prior to {DNA} replication at maturity and initiate their {DNA} replication only during fertilization. {M}ature male and female {A}rabidopsis gametes arrest their cell cycle before {DNA} replication, which only starts during fertilization.},
  keywords = {},
  booktitle = {},
  journal = {{P}lant {P}hysiology},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[10 p.]},
  ISSN = {0032-0889},
  year = {2023},
  DOI = {10.1093/plphys/kiad512},
  URL = {https://www.documentation.ird.fr/hor/fdi:010089654},
}