@article{fdi:010089513,
  title = {{E}ffects of mosquito age and batch size on knockdown and mortality of laboratory-reared {A}nopheles dirus, {A}nopheles minimus, and wild-caught {A}nopheles harrisoni ({D}iptera : {C}ulicidae) exposed to transfluthrin using {WHO} tube and {CDC} bottle bioassays},
  author = {{S}aeung, {M}. and {N}goen-{K}lan, {R}. and {Y}an, {C}. and {K}erdsawang, {J}. and {N}ararak, {J}. and {M}anguin, {S}ylvie and {H}ii, {J}. and {C}hareonviriyaphap, {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{WHO} tube and {CDC} bottle bioassays are currently available for insecticide resistance monitoring and malaria transmission research. {M}ultiple parameters including mosquito density, age, and nutritional status may affect the readout in these bioassays' tests. {T}his study aims to assess the effects of experimental factors on knockdown and mortality measurements in dominant malaria vectors in {T}hailand following exposure to sublethal and lethal doses of transfluthrin. {T}he effects of (i) 3 different mosquito batch sizes (5, 10, and 20 individuals) and (ii) 2 age groups (3-5 and 20-23 days old) on outcomes measured using the {WHO} tube (14.7 mu g/cm2) and {CDC} bottle bioassay discriminating concentration (0.006 mu g/cm2) against 2 laboratory strains: {A}nopheles dirus {P}eyton & {H}arrison and {A}nopheles minimus {T}heobald (species {A}) and wild-caught {A}nopheles harrisoni {H}arbach & {M}anguin (species {C}). {O}ur results showed higher knockdown at 1-h exposure using {WHO} tube and {CDC} bottle bioassays containing 20 individuals compared to batches containing 10 and 5 individuals. {O}lder mosquitoes showed greater susceptibility than younger test population, especially for {A}n. mininus. {O}ur study supports {WHO} recommendations for using 3- to 5-day-old mosquitoes. {I}t also validates proposal to divide the cohort into smaller batches with more test replicates when it is not practicable to test 25 mosquitoes per replicate.},
  keywords = {insecticide ; vector ; {A}nopheles ; malaria ; bioassays ; {THAILANDE}},
  booktitle = {},
  journal = {{J}ournal of {M}edical {E}ntomology},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[15 p.]},
  ISSN = {0022-2585},
  year = {2024},
  DOI = {10.1093/jme/tjae004},
  URL = {https://www.documentation.ird.fr/hor/fdi:010089513},
}