@article{fdi:010089489,
  title = {{M}atrix-assisted laser desorption/ionization time-of-flight mass spectrometry traces the geographical source of {B}iomphalaria pfeifferi and {B}ulinus forskalii, involved in schistosomiasis transmission},
  author = {{G}aye, {P}. {M}. and {N}diaye, {E}. {I}. and {D}oucour{\'e}, {S}ouleymane and {S}ow, {D}. and {G}aye, {M}. and {G}oumballa, {N}. and {C}assagne, {C}. and {L}'{O}llivier, {C}. and {M}ediannikov, {O}leg and {S}okhna, {C}heikh and {R}anque, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {F}reshwater snails of the genera {B}ulinus spp., {B}iomphalaria spp., and {O}ncomelania spp. are the main intermediate hosts of human and animal schistosomiasis. {I}dentification of these snails has long been based on morphological and/or genomic criteria, which have their limitations. {T}hese limitations include a lack of precision for the morphological tool and cost and time for the {DNA}-based approach. {R}ecently, {M}atrix-{A}ssisted {L}aser {D}esorption/{I}onization {T}ime-{O}f-{F}light ({MALDI}-{TOF}) mass spectrometry, a new tool used which is routinely in clinical microbiology, has emerged in the field of malacology for the identification of freshwater snails. {T}his study aimed to evaluate the ability of {MALDI}-{TOF} {MS} to identify {B}iomphalariapfeifferi and {B}ulinusforskalii snail populations according to their geographical origin. {M}ethods {T}his study was conducted on 101 {B}i.pfeifferi and 81 {B}u.forskalii snails collected in three distinct geographical areas of {S}enegal (the {N}orth-{E}ast, {S}outh-{E}ast and central part of the country), and supplemented with wild and laboratory strains. {S}pecimens which had previously been morphologically described were identified by {MALDI}-{TOF} {MS} [identification log score values ({LSV}) >= 1.7], after an initial blind test using the pre-existing database. {A}fter {DNA}-based identification, new reference spectra of {B}i.pfeifferi (n = 10) and {B}u.forskalii (n = 5) from the geographical areas were added to the {MALDI}-{TOF} spectral database. {T}he final blind test against this updated database was performed to assess identification at the geographic source level. {R}esults {MALDI}-{TOF} {MS} correctly identified 92.1% of 101 {B}i.pfeifferi snails and 98.8% of 81 {B}u.forskalii snails. {A}t the final blind test, 88% of 166 specimens were correctly identified according to both their species and sampling site, with {LSV}s ranging from 1.74 to 2.70. {T}he geographical source was adequately identified in 90.1% of 91 {B}i.pfeifferi and 85.3% of 75 {B}u.forskalii samples. {C}onclusions {O}ur findings demonstrate that {MALDI}-{TOF} {MS} can identify and differentiate snail populations according to geographical origin. {I}t outperforms the current {DNA}-based approaches in discriminating laboratory from wild strains. {T}his inexpensive high-throughput approach is likely to further revolutionise epidemiological studies in areas which are endemic for schistosomiasis.},
  keywords = {{M}atrix-assisted laser desorption/ionization time-of-flight mass spectrometry ; {S}nail ; {I}ntermediate host ; {B}iomphalariapfeifferi ; {B}ulinusforskalii ; {S}chistosomiasis ; {S}enegal ; {SENEGAL}},
  booktitle = {},
  journal = {{I}nfectious {D}iseases of {P}overty},
  volume = {13},
  numero = {1},
  pages = {11 [15 p.]},
  ISSN = {2095-5162},
  year = {2024},
  DOI = {10.1186/s40249-023-01168-y},
  URL = {https://www.documentation.ird.fr/hor/fdi:010089489},
}