Sondo M. auteur aut IRD Wonni I. auteur aut IRD Koïta K. auteur aut IRD Rimbault Isabelle auteur aut IRD Barro M. auteur aut IRD Tollenaere Charlotte auteur aut IRD Moulin Lionel auteur aut IRD Klonowska A. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Plant-associated bacteria are essential partners in plant health and development. In addition to taking advantage of the rapid advances recently achieved in high-throughput sequencing approaches, studies on plant-microbiome interactions require experiments with culturable bacteria. A study on the rice root microbiome was recently initiated in Burkina Faso. As a follow up, the aim of the present study was to develop a collection of corresponding rice root-associated bacteria covering maximum diversity, to assess the diversity of the obtained isolates based on the culture medium used, and to describe the taxonomy, phenotype and abundance of selected isolates in the rice microbiome. More than 3,000 isolates were obtained using five culture media (TSA, NGN, NFb, PCAT, Baz). The 16S rRNA fragment sequencing of 1,013 selected isolates showed that our working collection covered four bacterial phyla (Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes) and represented 33% of the previously described diversity of the rice root microbiome at the order level. Phenotypic in vitro analysis of the plant growth promoting capacity of the isolates revealed an overall ammonium production and auxin biosynthesis capacity, while siderophore production and phosphate solubilisation were enriched in Burkholderia, Ralstonia, Acinetobacter and Pseudomonas species. Of 45 representative isolates screened for growth promotion on seedlings of two rice cultivars, five showed an ability to improve the growth of both cultivars, while five others were effective on only one cultivar. The best results were obtained with Pseudomonas taiwanensis ABIP 2315 and Azorhizobium caulinodans ABIP 1219, which increased seedling growth by 158% and 47%, respectively. Among the 14 best performing isolates, eight appeared to be abundant in the rice root microbiome dataset from previous study. The findings of this research contribute to the in vitro and in planta PGP capacities description of rice root-associated bacteria and their potential importance for plants by providing, for the first time, insight into their prevalence in the rice root microbiome. specialized BURKINA FASO 076 084 18 11 e0287084 [27 ] 2023 1932-6203 https://www.documentation.ird.fr/hor/fdi:010089060 10.1371/journal.pone.0287084 1932-6203 [F B010089060] https://www.documentation.ird.fr/hor/fdi:010089060 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-01/010089060.pdf IRD - Base Horizon / Pleins textes 2024-01-04 2025-02-24 fdi:010089060 fre