@article{fdi:010089060,
  title = {{D}iversity and plant growth promoting ability of rice root-associated bacteria in {B}urkina-{F}aso and cross-comparison with metabarcoding data},
  author = {{S}ondo, {M}. and {W}onni, {I}. and {K}o{\¨ie}ta, {K}. and {R}imbault, {I}sabelle and {B}arro, {M}. and {T}ollenaere, {C}harlotte and {M}oulin, {L}ionel and {K}lonowska, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lant-associated bacteria are essential partners in plant health and development. {I}n addition to taking advantage of the rapid advances recently achieved in high-throughput sequencing approaches, studies on plant-microbiome interactions require experiments with culturable bacteria. {A} study on the rice root microbiome was recently initiated in {B}urkina {F}aso. {A}s a follow up, the aim of the present study was to develop a collection of corresponding rice root-associated bacteria covering maximum diversity, to assess the diversity of the obtained isolates based on the culture medium used, and to describe the taxonomy, phenotype and abundance of selected isolates in the rice microbiome. {M}ore than 3,000 isolates were obtained using five culture media ({TSA}, {NGN}, {NF}b, {PCAT}, {B}az). {T}he 16{S} r{RNA} fragment sequencing of 1,013 selected isolates showed that our working collection covered four bacterial phyla ({P}roteobacteria, {F}irmicutes, {A}ctinobacteria and {B}acteroidetes) and represented 33% of the previously described diversity of the rice root microbiome at the order level. {P}henotypic in vitro analysis of the plant growth promoting capacity of the isolates revealed an overall ammonium production and auxin biosynthesis capacity, while siderophore production and phosphate solubilisation were enriched in {B}urkholderia, {R}alstonia, {A}cinetobacter and {P}seudomonas species. {O}f 45 representative isolates screened for growth promotion on seedlings of two rice cultivars, five showed an ability to improve the growth of both cultivars, while five others were effective on only one cultivar. {T}he best results were obtained with {P}seudomonas taiwanensis {ABIP} 2315 and {A}zorhizobium caulinodans {ABIP} 1219, which increased seedling growth by 158% and 47%, respectively. {A}mong the 14 best performing isolates, eight appeared to be abundant in the rice root microbiome dataset from previous study. {T}he findings of this research contribute to the in vitro and in planta {PGP} capacities description of rice root-associated bacteria and their potential importance for plants by providing, for the first time, insight into their prevalence in the rice root microbiome.},
  keywords = {{BURKINA} {FASO}},
  booktitle = {},
  journal = {{P}lo{S} {O}ne},
  volume = {18},
  numero = {11},
  pages = {e0287084 [27 ]},
  ISSN = {1932-6203},
  year = {2023},
  DOI = {10.1371/journal.pone.0287084},
  URL = {https://www.documentation.ird.fr/hor/fdi:010089060},
}