Duffy antigen is expressed during erythropoiesis in Duffy-negative individuals /Dechavanne, Célia Dechavanne, S. Bosch, J. Metral, S. Redinger, K. R. Watson, Q. D. Ratsimbasoa, A. C. Roeper, B. Krishnan, S. Fong, R. Bennett, S. Carias, L. Chen, E. D. Salinas, N. D. Ghosh, A. Tolia, N. H. Woost, P. G. Jacobberger, J. W. Colin, Y. Gamain, B. King, C. L. Zimmerman, P. A. The erythrocyte silent Duffy blood group phenotype in Africans is thought to confer resistance to Plasmodium vivax blood-stage infection. However, recent studies report P. vivax infections across Africa in Fy-negative individuals. This suggests that the globin transcription factor 1 (GATA-1) SNP underlying Fy negativity does not entirely abolish Fy expression or that P. vivax has developed a Fy-independent red blood cell (RBC) invasion pathway. We show that RBCs and erythroid progenitors from in vitro differentiated CD34 cells and from bone marrow aspirates from Fy-negative samples express a functional Fy on their surface. This suggests that the GATA-1 SNP does not entirely abolish Fy expression. Given these results, we developed an in vitro culture system for P. vivax and show P. vivax can invade erythrocytes from Duffy-negative individuals. This study provides evidence that Fy is expressed in Fy-negative individuals and explains their susceptibility to P. vivax with major implications and challenges for P. vivax malaria eradication. 2023 text https://www.documentation.ird.fr/hor/fdi:010088919 fdi:010088919 Dechavanne Célia, Dechavanne S., Bosch J., Metral S., Redinger K. R., Watson Q. D., Ratsimbasoa A. C., Roeper B., Krishnan S., Fong R., Bennett S., Carias L., Chen E. D., Salinas N. D., Ghosh A., Tolia N. H., Woost P. G., Jacobberger J. W., Colin Y., Gamain B., King C. L., Zimmerman P. A.. Duffy antigen is expressed during erythropoiesis in Duffy-negative individuals. 2023, 31 (12), [22 p.] EN AFRIQUE