@article{fdi:010088905,
  title = {{C}ovariation between microeukaryotes and bacteria associated with {P}lanorbidae snails},
  author = {{C}lerissi, {C}. and {H}uot, {C}. and {P}ortet, {A}na{\¨ie}s and {G}ourbal, {B}. and {T}oulza, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}icrobial communities associated with macroorganisms might affect host physiology and homeostasis. {B}acteria are well studied in this context, but the diversity of microeukaryotes, as well as covariations with bacterial communities, remains almost unknown. {M}ethods: {T}o study microeukaryotic communities associated with {P}lanorbidae snails, we developed a blocking primer to reduce amplification of host {DNA} during metabarcoding analyses. {A}nalyses of alpha and beta diversities were computed to describe microeukaryotes and bacteria using metabarcoding of 18{S} and 16{S} r{RNA} genes, respectively. {R}esults: {O}nly three phyla ({A}moebozoa, {O}pisthokonta and {A}lveolata) were dominant for microeukaryotes. {B}acteria were more diverse with five dominant phyla ({P}roteobacteria, {B}acteroidetes, {T}enericutes, {P}lanctomycetes and {A}ctinobacteria). {T}he composition of microeukaryotes and bacteria were correlated for the {B}iomphalaria glabrata species, but not for {P}lanorbarius metidjensis. {N}etwork analysis highlighted clusters of covarying taxa. {A}mong them, several links might reflect top-down control of bacterial populations by microeukaryotes, but also possible competition between microeukaryotes having opposite distributions ({L}obosa and {I}chthyosporea). {T}he role of these taxa remains unknown, but we believe that the blocking primer developed herein offers new possibilities to study the hidden diversity of microeukaryotes within snail microbiota, and to shed light on their underestimated interactions with bacteria and hosts.},
  keywords = {{H}olobiont ; {M}icrobiota ; {M}etabarcoding ; {B}locking primer ; {H}eterobranchia},
  booktitle = {},
  journal = {{P}eer{J}},
  volume = {11},
  numero = {},
  pages = {e16639 [25 ]},
  ISSN = {2167-8359},
  year = {2023},
  DOI = {10.7717/peerj.16639},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088905},
}