@article{fdi:010088902,
  title = {{S}ource apportionment of oxidative potential depends on the choice of the assay : insights into 5 protocols comparison and implications for mitigation measures},
  author = {{D}ominutti, {P}. {A}. and {B}orlaza, {L}. {J}. {S}. and {S}auvain, {J}. {J}. and {T}huy, {V}. {D}. {N}. and {H}oudier, {S}. and {S}uarez, {G}. and {J}affrezo, {J}. {L}. and {T}obin, {S}. and {T}r{\'e}buchon, {C}. and {S}ocquet, {S}. and {M}oussu, {E}. and {M}ary, {G}. and {U}zu, {G}a{\¨e}lle},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he oxidative potential ({OP}) of particulate matter ({PM}) has recently been considered as a viable health-based metric of {PM} exposure. {S}everal acellular assays have been developed to assess {OP}, but there is no clear consensus in the methodology or protocols used that allows inter-comparison between studies. {T}his research investigates five different acellular {OP} assays and their sensitivities with the chemical composition of atmospheric particulate matter ({PM}10) and its emission sources. {W}e revisited and evaluated {PM}10 samples collected over one year in the urban {A}lpine city of {G}renoble, {F}rance. {T}he {OP} of {PM}10 was assessed by integrating assays commonly found in the literature, such as ascorbic acid ({AA}), dithiothreitol ({DTT}) and 2,7-dichlorofluorescein ({DCFH}) but also adding novel and less explored assays such as {F}erric-{X}ylenol {O}range ({FOX}), and a direct {ROS}-quantification through {OH}. {D}etailed source apportionment of {PM} using positive matrix factorisation ({PMF}) previously performed was coupled with multiple linear regression ({MLR}) models to determine the {OP} contribution of {PM}10 sources. {T}he results highlight the importance of seasonality in the mass contributions of each source and its corresponding influence on {OP}. {T}hese seasonal differences helped to identify the specific reactivity for each studied {OP} assay. {I}n winter, a good agreement was found between all the {OP} assays with anthropogenic sources. {H}owever, during warmer months, with a reduction in the share of anthropogenic emissions, a higher impact from biogenic and secondary organic-related aerosols has been found. {O}ur results also show a dissimilar sensitivity of each {OP} to the {PM}10 sources, likely associated with the chemical composition and chemical processes involved. {T}hus, our findings show the importance of combining various {OP} assays to capture different sensitivities to redox-active species to get a clearer picture of the intrinsic capacity of {PM} sources to cause damaging oxidative reactions in the lung. {P}roviding the heterogeneity of sources obtained with the different {OP} assays for a given ambient {PM} exposure, the choice of a single or a combination of {OP} method(s) must be rationally evaluated as part of the assessment strategy. {S}uch a choice would offer valuable source-related information as a powerful tool to better understand the nature and the intensity of air pollution and envisage the targeted sources for future mitigation policies.},
  keywords = {{FRANCE} ; {ALPES} ; {GRENOBLE}},
  booktitle = {},
  journal = {{E}nvironmental {S}cience : {A}tmospheres},
  volume = {3},
  numero = {10},
  pages = {1497--1512},
  year = {2023},
  DOI = {10.1039/d3ea00007a},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088902},
}