@article{fdi:010088882,
  title = {{B}othrops venom-induced hemostasis disorders in the rat : between {S}cylla and {C}harybdis},
  author = {{L}arr{\'e}ch{\'e}, {S}. and {C}hevillard, {L}. and {J}ourdi, {G}. and {M}ath{\'e}, {S}. and {S}ervonnet, {A}. and {J}oly, {B}. {S}. and {S}iguret, {V}. and {C}hippaux, {J}ean-{P}hilippe and {M}{\'e}garbane, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}emostasis impairment represents the most threatening consequence of {V}iperidae envenoming, notably with {B}othrops genus. {I}n the {F}rench departments of {A}merica, {B}. atrox envenomation in {F}rench {G}uiana may lead to bleeding while {B}. lanceolatus envenomation in {M}artinique to thrombosis. {B}leeding related to {B}. atrox envenomation is attributed to vascular damage mediated by venom metalloproteinases and blood uncoagulable state resulting from thrombocytopenia and consumptive coagulopathy. {T}hrombosis related to {B}. lanceolatus envenomation are poorly understood. {W}e aimed to compare the effects of {B}. atrox and {B}. lanceolatus venoms in the rat to identify the determinants of the hemorrhagic versus thrombotic complications. {V}iscoelastometry ({ROTEM}), platelet count, plasma fibrinogen, thrombin generation assay, fibrinography, endothelial (von {W}illebrand factor, {ADAMTS}13 activity, {ICAM}-1, and soluble {E}-selectin), and inflammatory biomarkers ({IL}-1 beta, {IL}-6, {TNF}-alpha, {MCP}-1, and {PAI}-1) were determined in blood samples obtained at {H}3, {H}6, and {H}24 after the subcutaneous venom versus saline injection. {I}n comparison to the control, initial fibrinogen consumption was observed with the two venoms while thrombocytopenia and reduction in the clot amplitude only with {B}. atrox venom. {M}oreover, we showed an increase in thrombin generation at {H}3 with the two venoms, an increase in fibrin generation accompanied with hyperfibrinogenemia at {H}24 and an increase in inflammatory biomarkers with {B}. lanceolatus venom. {N}o endothelial damage was found with the two venoms. {T}o conclude, our data support two-sided hemostasis complications in {B}othrops envenoming with an initial risk of hemorrhage related to platelet consumption and hypocoagulability followed by an increased risk of thrombosis promoted by the activated inflammatory response and rapid-onset fibrinogen restoration.},
  keywords = {{GUYANE} {FRANCAISE} ; {MARTINIQUE}},
  booktitle = {},
  journal = {{PL}o{S} {N}eglected {T}ropical {D}iseases},
  volume = {17},
  numero = {11},
  pages = {e0011786 [21 p.]},
  ISSN = {1935-2735},
  year = {2023},
  DOI = {10.1371/journal.pntd.0011786},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088882},
}