@article{fdi:010088845,
  title = {{M}orphological, molecular, and {MALDI}-{TOF} {MS} identification of bed bugs and associated {W}olbachia species from {C}ameroon},
  author = {{N}gnindji-{Y}oudje, {Y}. and {L}ontsi-{D}emano, {M}. and {D}iarra, {A}dama {Z}an and {M}akaila, {A}. {M}. and {B}erenger, {J}. {M}. and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}fter vanishing from the public eye for more than 50 years, bed bugs have resurged to become one of the most widely discussed and heavily researched insect pests in the world. {T}his study presents the basic information of infestations of tropical bed bugs, {C}imex hemipterus ({H}emiptera: {C}imicidae), in {C}ameroon. {A} total of 248 immature stage and adult bed bug specimens were collected from households and a travel agency in {Y}aounde ' and {D}ouala, {C}ameroon. {T}he ability of {MALDI}-{TOF} {MS} to identify bed bugs was tested using heads for adults and cephalo-thoraxes for immature stages. {M}icroorganism screening was performed by q{PCR} and confirmed by regular {PCR} and sequencing. {B}ased on morphometrical criteria, four stages of immature bed bugs are represented. {O}f the 248 bed bug specimens morphologically identified as {C}imex hemipterus , 246 (77 males, 65 females and 104 immature specimens) were submitted to {MALDI}-{TOF} {MS} analysis. {O}f the 222 adults and immature specimens tested, 122 (59.9 %) produced good quality {MALDI}-{TOF} {MS} spectra (35 adults and 87 immature specimens). {B}lind testing allowed species level identification of 98.21 % of adult and immature {C}. hemipterus. {A}mong the bacteria tested, only {W}olbachia {DNA} was found in 12/246 (4.8 %) bed bugs. {M}ore surveys in the country are warranted to assess the true level of bed bug infestations, in order to take appropriate action for their control.},
  keywords = {{B}ed bugs ; {C}imex hemipterus ; {MALDI}-{TOF} {MS} ; {W}olbachia ; {C}ameroon ; {CAMEROUN}},
  booktitle = {},
  journal = {{A}cta {T}ropica},
  volume = {249},
  numero = {},
  pages = {107086 [9 ]},
  ISSN = {0001-706{X}},
  year = {2024},
  DOI = {10.1016/j.actatropica.2023.107086},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088845},
}