@article{fdi:010088843,
  title = {{R}ice {Y}ellow {M}ottle {V}irus resistance by genome editing of the {O}ryza sativa {L}. ssp. japonica nucleoporin gene {O}s{CPR}5.1 but not {O}s{CPR}5.2},
  author = {{A}rra, {Y}. and {A}uguy, {F}lorence and {S}tiebner, {M}. and {C}h{\'e}ron, {S}ophie and {W}udick, {M}. {M}. and {M}iras, {M}. and {S}chepler-{L}uu, {V}. and {K}{\¨o}hler, {S}. and {C}unnac, {S}{\'e}bastien and {F}rommer, {W}. {B}. and {A}lbar, {L}aurence},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice yellow mottle virus ({RYMV}) causes one of the most devastating rice diseases in {A}frica. {M}anagement of {RYMV} is challenging. {G}enetic resistance provides the most effective and environment-friendly control. {T}he recessive resistance locus rymv2 ({O}s{CPR}5.1) had been identified in {A}frican rice ({O}ryza glaberrima), however, introgression into {O}ryza sativa ssp. japonica and indica remains challenging due to crossing barriers. {H}ere, we evaluated whether {CRISPR}/{C}as9 genome editing of the two rice nucleoporin paralogs {O}s{CPR}5.1 ({RYMV}2) and {O}s{CPR}5.2 can be used to introduce {RYMV} resistance into the japonica variety {K}itaake. {B}oth paralogs had been shown to complement the defects of the {A}rabidopsis atcpr5 mutant, indicating partial redundancy. {D}espite striking sequence and structural similarities between the two paralogs, only oscpr5.1 loss-of-function mutants were fully resistant, while loss-of-function oscpr5.2 mutants remained susceptible, intimating that {O}s{CPR}5.1 plays a specific role in {RYMV} susceptibility. {N}otably, edited lines with short in-frame deletions or replacements in the {N}-terminal domain (predicted to be unstructured) of {O}s{CPR}5.1 were hypersusceptible to {RYMV}. {I}n contrast to mutations in the single {A}rabidopsis {A}t{CPR}5 gene, which caused severely dwarfed plants, oscpr5.1 and oscpr5.2 single and double knockout mutants showed neither substantial growth defects nor symptoms indicative lesion mimic phenotypes, possibly reflecting functional differentiation. {T}he specific editing of {O}s{CPR}5.1, while maintaining {O}s{CPR}5.2 activity, provides a promising strategy for generating {RYMV}-resistance in elite {O}ryza sativa lines as well as for effective stacking with other {RYMV} resistance genes or other traits.},
  keywords = {genome editing ; {CRISPR}/{C}as9 ; knockout mutant ; disease resistance ; recessive ; nucleoporin},
  booktitle = {},
  journal = {{P}lant {B}iotechnology {J}ournal},
  volume = {{E}arly access},
  numero = {},
  pages = {[13 ]},
  ISSN = {1467-7644},
  year = {2023},
  DOI = {10.1111/pbi.14266},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088843},
}