%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Abou Chacra, L.
%A Benatmane, A.
%A Iwaza, R.
%A Ly, C.
%A Alibar, S.
%A Armstrong, N.
%A Mediannikov, Oleg
%A Bretelle, F.
%A Fenollar, F.
%T Culturomics reveals a hidden world of vaginal microbiota with the isolation of 206 bacteria from a single vaginal sample
%D 2024
%L fdi:010088807
%G ENG
%J Archives of Microbiology
%@ 0302-8933
%K Vaginal microbiota ; Anaerobes ; Culturomics ; Culture conditions ; MALDI-TOF ; Porphyromonas vaginalis
%M ISI:001125123700002
%N 1
%P 20 [12 ]
%R 10.1007/s00203-023-03742-2
%U https://www.documentation.ird.fr/hor/fdi:010088807
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-01/010088807.pdf
%V 206
%W Horizon (IRD)
%X The composition of the vaginal microbiota is known to be influenced by various factors and to be associated with several disorders affecting women's health. Although metagenomics is currently a widely used method for studying the human microbiota, it has certain limitations, such as a lack of information on bacterial viability. It is therefore important to use culture-based methods such as culturomics. Here, we used 35 different culture conditions to comprehensively characterize the vaginal bacterial diversity of a single woman's flora. A total of 206 bacterial species, belonging to six phyla (for a little more than half to Firmicutes, followed mainly by Actinobacteria, Bacteroidetes, and Proteobacteria) and 45 families, and 2 fungal species were cultivated. While several species of lactobacilli have been isolated, a wide variety of other bacteria were also separated, including 65 never reported before in vaginal flora, including a new bacterial species, Porphyromonas vaginalis sp. nov. Extensive culture-based methods are essential to establish a comprehensive, evidence-based repertoire of bacterial viability. If combined with molecular methods, they can provide a much more thorough understanding of the vaginal microbiota and fulfil the unknown part of metagenomic studies.
%$ 084 ; 050