@article{fdi:010088807,
  title = {{C}ulturomics reveals a hidden world of vaginal microbiota with the isolation of 206 bacteria from a single vaginal sample},
  author = {{A}bou {C}hacra, {L}. and {B}enatmane, {A}. and {I}waza, {R}. and {L}y, {C}. and {A}libar, {S}. and {A}rmstrong, {N}. and {M}ediannikov, {O}leg and {B}retelle, {F}. and {F}enollar, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he composition of the vaginal microbiota is known to be influenced by various factors and to be associated with several disorders affecting women's health. {A}lthough metagenomics is currently a widely used method for studying the human microbiota, it has certain limitations, such as a lack of information on bacterial viability. {I}t is therefore important to use culture-based methods such as culturomics. {H}ere, we used 35 different culture conditions to comprehensively characterize the vaginal bacterial diversity of a single woman's flora. {A} total of 206 bacterial species, belonging to six phyla (for a little more than half to {F}irmicutes, followed mainly by {A}ctinobacteria, {B}acteroidetes, and {P}roteobacteria) and 45 families, and 2 fungal species were cultivated. {W}hile several species of lactobacilli have been isolated, a wide variety of other bacteria were also separated, including 65 never reported before in vaginal flora, including a new bacterial species, {P}orphyromonas vaginalis sp. nov. {E}xtensive culture-based methods are essential to establish a comprehensive, evidence-based repertoire of bacterial viability. {I}f combined with molecular methods, they can provide a much more thorough understanding of the vaginal microbiota and fulfil the unknown part of metagenomic studies.},
  keywords = {{V}aginal microbiota ; {A}naerobes ; {C}ulturomics ; {C}ulture conditions ; {MALDI}-{TOF} ; {P}orphyromonas vaginalis},
  booktitle = {},
  journal = {{A}rchives of {M}icrobiology},
  volume = {206},
  numero = {1},
  pages = {20 [12 p.]},
  ISSN = {0302-8933},
  year = {2024},
  DOI = {10.1007/s00203-023-03742-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088807},
}