@article{fdi:010088792,
  title = {{P}lasmodium falciparum population structure and genetic diversity of cell traversal protein for ookinetes and sporozoites ({C}el{TOS}) during malaria resurgences in {D}ielmo, {S}enegal},
  author = {{W}otodjo, {A}. {N}. and {O}boh, {M}. {A}. and {S}okhna, {C}heikh and {D}iagne, {N}afissatou and {D}iene-{S}arr, {F}. and {T}rape, {J}ean-{F}ran{\c{c}}ois and {D}oucour{\'e}, {S}ouleymane and {A}mambua-{N}gwa, {A}. and {D}'{A}lessandro, {U}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he ability to accurately measure the intensity of malaria transmission in areas with low transmission is extremely important to guide elimination efforts. {P}lasmodium falciparum {C}ell-traversal protein for ookinetes and sporozoites ({P}f{C}el{TOS}) is an important conserved sporozoite antigen reported as one of the promising malaria vaccine candidates, and could be used to estimate malaria transmission intensity. {T}his study aimed at deter-mining whether the diversity of {P}f{C}el{TOS} gene reflects the changes in malaria transmission that occurred be-tween 2007 and 2014 in {D}ielmo, a {S}enegalese village, before and after the implementation of insecticide treated bed nets ({ITN}s). {O}f the 109 samples positive for {P}f{C}el{TOS} {PCR}, 96 (88%) were successfully sequenced and analysed for polymorphisms and population diversity. {T}he number of segregating sites was higher during the pre-intervention period (13) and the malaria resurgences (11) than during the intervention period (5). {S}imilarly, the number and diversity of haplotypes were higher during the pre-intervention period (16 and 0.914, respectively) and the malaria resurgences (6 and 0.821, respectively) than during the intervention period (4 and 0.758, respectively). {M}oreover, the average number of nucleotide differences was higher during the pre-intervention (3.792) and during malaria resurgences (3.467) than during the intervention period (2.189). {T}he 3{D}7 {KSSFNEP} haplotype was only observed during the intervention period. {O}nly two haplotypes were shared in both the pre-intervention and intervention periods while four haplotypes were shared between the pre-intervention and the malaria resurgences. {T}he {F}st values indicate moderate differentiation between pre-intervention and intervention periods (0.17433), and between intervention and malaria resurgences period (0.19198) as well as between pre-intervention and malaria resurgences periods (0.06607).{P}f{C}el{TOS} genetic diversity reflected changes of malaria transmission, with higher polymorphisms recorded before the large-scale implementation of {ITN}s and during the malaria resurgences. {P}f{C}el{TOS} is also a candidate vaccine; mapping its diversity across multiple endemic environments will facilitate the design and optimisation of a broad and efficacious vaccine.},
  keywords = {{P}f{C}el{TOS} ; {M}alaria transmission ; {M}alaria resurgences ; {P}opulation structure ; {G}enetic diversity ; {S}enegal ; {SENEGAL}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {116},
  numero = {},
  pages = {105535 [7 ]},
  ISSN = {1567-1348},
  year = {2023},
  DOI = {10.1016/j.meegid.2023.105535},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088792},
}