@article{fdi:010088772,
  title = {{C}haracterization of the genetic composition and establishment of a core collection for the {INERA} {R}obusta coffee ({C}offea canephora) field genebank from the {D}emocratic {R}epublic of {C}ongo},
  author = {{V}erleysen, {L}. and {B}ollen, {R}. and {K}ambale, {J}. {L}. and {E}bele, {T}. and {K}atshela, {B}. {N}. and {D}epecker, {J}. and {P}oncet, {V}al{\'e}rie and {A}ssumani, {D}. {M}. and {V}andelook, {F}. and {S}toffelen, {P}. and {H}onnay, {O}. and {R}uttink, {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ultivation of {R}obusta coffee is likely to gain importance because of its high disease resistance and climate envelope. {R}obusta coffee genetic resources conserved in field genebanks can play an important role to further improve its cupping quality and other agronomic traits, but such {C}offea canephora collections are limited and still poorly characterized. {I}n this study, we characterized the genetic composition of the historically important but until recently neglected {INERA} {C}offee {C}ollection in {Y}angambi (the {D}emocratic {R}epublic of {C}ongo). {W}e used {GBS} to discover genome-wide genetic diversity, created and validated a novel multiplex amplicon sequencing ({H}i{P}lex) screening assay to genetically screen 730 coffee shrubs of the {Y}angambi {C}offee {C}ollection, grouped clonal material and delineated 263 accessions with unique genetic fingerprints. {C}omparison to reference material of three genetic origins revealed that the majority of the {Y}angambi accessions were assigned a '{L}ula' cultivar origin, four accessions were assigned to {C}ongolese subgroup {A} and nine accessions were most closely related to local wild accessions. {A}bout one-quarter of the accessions was likely derived from hybridization between these groups, which could result from seed-based propagation of the collection, breeding efforts, or natural cross-pollination. {P}arental analyses discovered eight preferentially used accessions, which may correspond to historically selected founders, or direct descendants thereof, whose seed material was once widely used to establish coffee plantations. {F}inally, two core collections were proposed using the maximization strategy ({CC}-{I}; 100 accessions) and genetic distance method ({CC}-{X}; 10 accessions). {O}ur study demonstrates a method for the genetic characterization of {R}obusta coffee collections in general and contributes to the re-evaluation and exploration of the {R}obusta coffee genetic resources in the {D}emocratic {R}epublic of the {C}ongo in particular.},
  keywords = {{C}offea canephora ; {H}i{P}lex amplicon sequencing ; genetic structure ; core collection ; ex-situ conservation ; crop wild relatives ; {D}emocratic {R}epublic of {C}ongo ; {REPUBLIQUE} {DEMOCRATIQUE} {DU} {CONGO}},
  booktitle = {},
  journal = {{F}rontiers in {S}ustainable {F}ood {S}ystems},
  volume = {7},
  numero = {},
  pages = {1239442 [17 p.]},
  year = {2023},
  DOI = {10.3389/fsufs.2023.1239442},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088772},
}