Hongjaisee S. auteur aut IRD Kham-Kjing N. auteur aut IRD Musikul P. auteur aut IRD Daengkaokhew W. auteur aut IRD Kongson N. auteur aut IRD Guntala R. auteur aut IRD Jaiyapan N. auteur aut IRD Kline E. auteur aut IRD Panpradist N. auteur aut IRD Ngo-Giang-Huong Nicole auteur aut IRD Khamduang W. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Since SARS-CoV-2 is a highly transmissible virus, a rapid and accurate diagnostic method is necessary to prevent virus spread. We aimed to develop and evaluate a new rapid colorimetric reverse transcription loop--mediated isothermal amplification (RT-LAMP) assay for SARS-CoV-2 detection in a single closed tube. Nasopharyngeal and throat swabs collected from at-risk individuals testing for SARS-CoV-2 were used to assess the sensitivity and specificity of a new RT-LAMP assay against a commercial qRT-PCR assay. Total RNA extracts were submitted to the RT-LAMP reaction under optimal conditions and amplified at 65 degrees C for 30 min using three sets of specific primers targeting the nucleocapsid gene. The reaction was detected using two different indicator dyes, hydroxynaphthol blue (HNB) and cresol red. A total of 82 samples were used for detection with HNB and 94 samples with cresol red, and results were compared with the qRT-PCR assay. The sensitivity of the RT-LAMP-based HNB assay was 92.1% and the specificity was 93.2%. The sensitivity of the RT-LAMP-based cresol red assay was 80.3%, and the specificity was 97%. This colorimetric feature makes this assay highly accessible, low-cost, and user-friendly, which can be deployed for massive scale-up and rapid diagnosis of SARS-CoV-2 infection, particularly in low-resource settings. specialized COVID-19 SARS-CoV-2 N gene RT-LAMP hydroxynaphthol blue cresol red THAILANDE 020 052 13 19 3040 [8 p.] 2023 https://www.documentation.ird.fr/hor/fdi:010088768 10.3390/diagnostics13193040 [F B010088768] https://www.documentation.ird.fr/hor/fdi:010088768 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-01/010088768.pdf IRD - Base Horizon / Pleins textes 2024-01-12 2025-02-24 fdi:010088768 fre