%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Hongjaisee, S.
%A Kham-Kjing, N.
%A Musikul, P.
%A Daengkaokhew, W.
%A Kongson, N.
%A Guntala, R.
%A Jaiyapan, N.
%A Kline, E.
%A Panpradist, N.
%A Ngo-Giang-Huong, Nicole
%A Khamduang, W.
%T A single-tube colorimetric loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 RNA
%D 2023
%L fdi:010088768
%G ENG
%J Diagnostics
%K COVID-19 ; SARS-CoV-2 N gene ; RT-LAMP ; hydroxynaphthol blue ; cresol red
%K THAILANDE
%M ISI:001122377400001
%N 19
%P 3040 [8 ]
%R 10.3390/diagnostics13193040
%U https://www.documentation.ird.fr/hor/fdi:010088768
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-01/010088768.pdf
%V 13
%W Horizon (IRD)
%X Since SARS-CoV-2 is a highly transmissible virus, a rapid and accurate diagnostic method is necessary to prevent virus spread. We aimed to develop and evaluate a new rapid colorimetric reverse transcription loop--mediated isothermal amplification (RT-LAMP) assay for SARS-CoV-2 detection in a single closed tube. Nasopharyngeal and throat swabs collected from at-risk individuals testing for SARS-CoV-2 were used to assess the sensitivity and specificity of a new RT-LAMP assay against a commercial qRT-PCR assay. Total RNA extracts were submitted to the RT-LAMP reaction under optimal conditions and amplified at 65 degrees C for 30 min using three sets of specific primers targeting the nucleocapsid gene. The reaction was detected using two different indicator dyes, hydroxynaphthol blue (HNB) and cresol red. A total of 82 samples were used for detection with HNB and 94 samples with cresol red, and results were compared with the qRT-PCR assay. The sensitivity of the RT-LAMP-based HNB assay was 92.1% and the specificity was 93.2%. The sensitivity of the RT-LAMP-based cresol red assay was 80.3%, and the specificity was 97%. This colorimetric feature makes this assay highly accessible, low-cost, and user-friendly, which can be deployed for massive scale-up and rapid diagnosis of SARS-CoV-2 infection, particularly in low-resource settings.
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