Human muscle cells sensitivity to chikungunya virus infection relies on their glycolysis activity and differentiation stage

Human muscle cells sensitivity to chikungunya virus infection relies on their glycolysis activity and differentiation stage Jaquet M. auteur aut IRD Bengue Michèle auteur aut IRD Lambert K. auteur aut IRD Carnac G. auteur aut IRD Missé Dorothée auteur aut IRD Bisbal C. auteur aut IRD text journalArticle eng text/pdf born digital access Changes to our environment have led to the emergence of human pathogens such as chikungunya virus. Chikungunya virus infection is today a major public health concern. It is a debilitating chronic disease impeding patients' mobility, affecting millions of people. Disease development relies on skeletal muscle infection. The importance of skeletal muscle in chikungunya virus infection led to the hypothesis that it could serve as a viral reservoir and could participate to virus persistence. Here we questioned the interconnection between skeletal muscle cells metabolism, their differentiation stage and the infectivity of the chikungunya virus. We infected human skeletal muscle stem cells at different stages of differentiation with chikungunya virus to study the impact of their metabolism on virus production and inversely the impact of virus on cell metabolism. We observed that chikungunya virus infectivity is cell differentiation and metabolism-dependent. Chikungunya virus interferes with the cellular metabolism in quiescent undifferentiated and proliferative muscle cells. Moreover, activation of chikungunya infected quiescent muscle stem cells, induces their proliferation, increases glycolysis and amplifies virus pro-duction. Therefore, our results showed that Chikungunya virus infectivity and the antiviral response of skeletal muscle cells relies on their energetic metabolism and their differentiation stage. Then, muscle stem cells could serve as viral reservoir producing virus after their activation. specialized Human skeletal muscle Metabolism Viral infection Chikungunya virus Viral reservoir Stem cells 020 052 Biochimie 218 85-95 2024 0300-9084 https://www.documentation.ird.fr/hor/fdi:010088763 10.1016/j.biochi.2023.09.005 0300-9084 [F B010088763] https://www.documentation.ird.fr/hor/fdi:010088763 https://www.documentation.ird.fr/intranet/publi/2024-01/010088763.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2024-01-12 2026-06-12 fdi:010088763 fre