@article{fdi:010088645,
  title = {{U}sing {MALDI}-{TOF} {MS} to identify mosquitoes from {S}enegal and the origin of their blood meals},
  author = {{F}all, {F}. {K}. and {D}iarra, {A}dama {Z}an and {B}ouganali, {C}harles and {S}okhna, {C}heikh and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}osquitoes are arthropods that represent a real public health problem in {A}frica. {M}orphology and molecular biology techniques are usually used to identify different mosquito species. {I}n recent years, an innovative tool, matrix-assisted desorption/ionization time-of-flight mass spectrometry ({MALDI}-{TOF} {MS}), has been used to identify many arthropods quickly and at low cost, where equipment is available. {W}e evaluated the ability of {MALDI}-{TOF} {MS} to identify mosquitoes collected in {S}enegal and stored for several months in silica gel, and to determine the origin of their blood meal. {A} total of 582 mosquitoes were collected and analysed. {W}e obtained 329/582 (56.52%) {MALDI}-{TOF} {MS} good-quality spectra from mosquito legs and 123/157 (78.34%) good-quality spectra from engorged abdomens. {W}e updated our home-made {MALDI}-{TOF} {MS} arthropod spectra database by adding 23 spectra of five mosquito species from {S}enegal that had been identified morphologically and molecularly. {T}hese included legs from {A}nopheles gambiae, {A}nopheles arabiensis, {A}nopheles cf. rivulorum, {C}ulex nebulosus, {A}nopheles funestus, and three spectra from abdomens engorged with human blood. {H}aving updated the database, all mosquitoes tested by {MALDI}-{TOF} {MS} were identified with scores greater than or equal to 1.7 as {A}n. gambiae (n = 64), {A}nopheles coluzzii (n = 12), {A}n. arabiensis (n = 1), {A}n. funestus (n = 7), {A}n. cf rivulorum (n = 1), {L}utzia tigripes (n = 3), {C}x. nebulosus (n = 211), {C}ulex quinquefasciatus (n = 2), {C}ulex duttoni (n = 1), {C}ulex perfescus (n = 1), {C}ulex tritaeniorhynchus (n = 1), and {A}edes aegypti (n = 2). {B}lood meal identification by {MALDI}-{TOF} {MS} revealed that mosquitoes had fed on the blood of humans (n = 97), cows (n = 6), dogs (n = 2), goats (n = 1), sheep (n = 1), and bats (n = 1). {M}ixed meals were also detected. {T}hese results confirm that {MALDI}-{TOF} {MS} is a promising technique for identifying mosquitoes and the origin of their blood meal.},
  keywords = {{MALDI}-{TOF} {MS} ; mosquitoes ; {A}nopheles ; {C}ulex ; {A}edes ; {S}enegal ; {SENEGAL}},
  booktitle = {},
  journal = {{I}nsects},
  volume = {14},
  numero = {10},
  pages = {785 [13 ]},
  year = {2023},
  DOI = {10.3390/insects14100785},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088645},
}