Seppey C. V. W. auteur aut IRD Cabrol Léa auteur aut IRD Thalasso F. auteur aut IRD Gandois L. auteur aut IRD Lavergne C. auteur aut IRD Martinez-Cruz K. auteur aut IRD Sepulveda-Jauregui A. auteur aut IRD Aguilar-Muñoz P. auteur aut IRD Astorga-España M. S. auteur aut IRD Chamy R. auteur aut IRD Dellagnezze B. M. auteur aut IRD Etchebehere C. auteur aut IRD Fochesatto G. J. auteur aut IRD Gerardo-Nieto O. auteur aut IRD Mansilla A. auteur aut IRD Murray A. auteur aut IRD Sweetlove M. auteur aut IRD Tananaev N. auteur aut IRD Teisserenc R. auteur aut IRD Tveit A. T. auteur aut IRD van de Putte A. auteur aut IRD Svenning M. M. auteur aut IRD Barret M. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Methane-cycling is becoming more important in high-latitude ecosystems as global warming makes permafrost organic carbon increasingly available. We explored 387 samples from three high-latitudes regions (Siberia, Alaska and Patagonia) focusing on mineral/organic soils (wetlands, peatlands, forest), lake/pond sediment and water. Physicochemical, climatic and geographic variables were integrated with 16S rDNA amplicon sequences to determine the structure of the overall microbial communities and of specific methanogenic and methanotrophic guilds. Physicochemistry (especially pH) explained the largest proportion of variation in guild composition, confirming species sorting (i.e., environmental filtering) as a key mechanism in microbial assembly. Geographic distance impacted more strongly beta diversity for (i) methanogens and methanotrophs than the overall prokaryotes and, (ii) the sediment habitat, suggesting that dispersal limitation contributed to shape the communities of methane-cycling microorganisms. Bioindicator taxa characterising different ecological niches (i.e., specific combinations of geographic, climatic and physicochemical variables) were identified, highlighting the importance of Methanoregula as generalist methanogens. Methylocystis and Methylocapsa were key methanotrophs in low pH niches while Methylobacter and Methylomonadaceae in neutral environments. This work gives insight into the present and projected distribution of methane-cycling microbes at high latitudes under climate change predictions, which is crucial for constraining their impact on greenhouse gas budgets. specialized RUSSIE ETATS UNIS CHILI ALASKA 082 084 021 [Early access] [23 ] 2023 1462-2912 https://www.documentation.ird.fr/hor/fdi:010088623 10.1111/1462-2920.16526 1462-2912 [F B010088623] https://www.documentation.ird.fr/hor/fdi:010088623 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-12/010088623.pdf IRD - Base Horizon / Pleins textes 2023-12-15 2025-02-24 fdi:010088623 fre