@article{fdi:010088606,
  title = {{P}hytoplanktonic species in the haloalkaline {L}ake {D}ziani {D}zaha select their archaeal microbiome},
  author = {{B}ruto, {M}. and {O}ger, {P}. {M}. and {G}ot, {P}. and {B}ernard, {C}. and {M}elayah, {D}. and {C}loarec, {L}. {A}. and {D}uval, {C}. and {E}scalas, {A}. and {D}uperron, {S}. and {G}uigard, {L}. and {L}eboulanger, {C}hristophe and {A}der, {M}. and {S}arazin, {G}. and {J}{\'e}z{\'e}quel, {D}. and {A}gogu{\'e}, {H}. and {T}roussellier, {M}. and {H}ugoni, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}icroorganisms are key contributors of aquatic biogeochemical cycles but their microscale ecology remains largely unexplored, especially interactions occurring between phytoplankton and microorganisms in the phycosphere, that is the region immediately surrounding phytoplankton cells. {T}he current study aimed to provide evidence of the phycosphere taking advantage of a unique hypersaline, hyperalkaline ecosystem, {L}ake {D}ziani {D}zaha ({M}ayotte), where two phytoplanktonic species permanently co-dominate: a cyanobacterium, {A}rthrospira fusiformis, and a green microalga, {P}icocystis salinarum. {T}o assay phycospheric microbial diversity from in situ sampling, we set up a flow cytometry cell-sorting methodology for both phytoplanktonic populations, coupled with metabarcoding and comparative microbiome diversity. {W}e focused on archaeal communities as they represent a non-negligible part of the phycospheric diversity, however their role is poorly understood. {T}his work is the first which successfully explores in situ archaeal diversity distribution showing contrasted phycospheric compositions, with {P}. salinarum phycosphere notably enriched in {W}oesearchaeales {OTU}s while {A}. fusiformis phycosphere was enriched in methanogenic lineages affiliated {OTU}s such as {M}ethanomicrobiales or {M}ethanofastidiosales. {M}ost archaeal {OTU}s, including {W}oesearchaeales considered in literature as symbionts, were either ubiquitous or specific of the free-living microbiome (i.e. present in the 3-0.2 mu m fraction). {S}eminally, several archaeal {OTU}s were enriched from the free-living microbiome to the phytoplankton phycospheres, suggesting (i) either the inhibition or decrease of other {OTU}s, or (ii) the selection of specific {OTU}s resulting from the physical influence of phytoplanktonic species on surrounding {A}rchaea.},
  keywords = {archaea ; microbial diversity ; microscale ; phycosphere ; thalassohaline ; ecosystem ; {OCEAN} {INDIEN} {ILES} ; {COMORES}},
  booktitle = {},
  journal = {{M}olecular {E}cology},
  numero = {},
  pages = {[15 p.]},
  ISSN = {0962-1083},
  year = {2023},
  DOI = {10.1111/mec.17179},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088606},
}