@article{fdi:010088493,
  title = {{A}ntibiotic resistance and virulence factors of extended-spectrum beta-lactamase-producing {K}lebsiella {P}neumoniae involved in healthcare-associated infections in {D}akar, {S}enegal},
  author = {{N}diaye, {I}. and {B}a, {B}.{S}. and {T}hiam, {F}. and {B}oye, {M}.{M}. and {S}ow, {O}. and {C}iss{\'e}, {A}. and {N}diaye, {B}. and {D}iallo, {T}.{A}. and {F}all, {C}. and {D}ieye, {Y}. and {D}i{\`e}ye, {B}. and {D}ieng, {A}. and {D}iop, {A}. and {C}onstantin de {M}agny, {G}uillaume and {S}eck, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {V}irulent and resistant {K}lebsiella pneumoniae strains are considered as one of the most significant causes of healthcare-associated infections ({HAI}s). {T}he aim of this study was to investigate the phenotypic and genotypic factors of antibiotic resistance and virulence factors of {ESBL} {K}. pneumoniae strains isolated from healthcare-associated infections ({HAI}s) in {D}akar, {S}enegal. {M}ethods: {T}wenty-eight strains of {K}. pneumoniae isolated from {HAI}s were collected from 2018 to 2021 in 2 main hospitals in {D}akar. {A}ntibiotic susceptibility and molecular characterization were studied using disk diffusion by the {K}irby-{B}auer method and {PCR}, respectively. {V}irulence factors were also determined by {PCR}. {R}esults: {T}hese {ESBL} {K}. pneumoniae isolates showed high resistance to antibiotics such as b{\^e}ta-lactams, aminoglycosides, cyclins, fluoroquinolones and trimethoprim-sulfamethoxazole. {A}mong these strains, ten (10) were resistant to carbapenem and cefoxitin (17.8%, n=5), chloramphenicol (25%, n=7) and fosfomycin (28.5%, n=8) considered as the most active antibiotics against {ESBL}-{KP} isolates. {E}ighteen (18) strains were considered as {MDR} and ten (10) strains as {XDR}. {F}or the genes associated to phenotypic resistance, b{\^e}ta-lactams resistance
was conferred through bla {SHV} (24/28), bla {TEM} (20/28) and mainly by bla{CTX}-{M}. {A}ll strains carried the bla{CTX}-{M}15 gene. {OXA}-48 (6/28) gene was found responsible for carbapenem resistance and other genes like {IMP}, {VIM}, {NDM}, {OXA}-23 were not detected. {P}lasmids-mediated resistance genes qnr{B} (16/28), qnr{S} (11/28) and aac(6')-{I}b (21/28), were mostly responsible for resistance to fluoroquinolones and aminoglycosides. {A}lso 3 of 6 virulence genes searched that are the most associated to the pathogenicity of {K}lebsiella pneumoniae were found on these strains with uge (19/28), mr{KD} (21/28) and fyu{A} (13/28).



{C}onclusion: {T}he {ESBL} {K}. pneumoniae strains isolated in this study showed a high prevalence of antibiotic resistance and virulence genes {T}he combination of these factors poses a potential risk for infections that could be highly virulent and difficult to treat. {T}hese findings demonstrated the importance of closely monitoring the resistance patterns of {K}. pneumoniae in hospitals seetings and emphasize the need to monitor effective antibiotic treatments for {K}. pneumoniae infections. {A}ditionnally, the scarcity of available data on {HAI}s, especially the prevalence of {M}ultidrug resistance bacteria and virulence factors associated with these {HAI}s in {S}enegal, further emphasizes the significance of implementing surveillance programs to better know their prevalence, impact on patient health and on length of hospital stays.},
  keywords = {{SENEGAL} ; {DAKAR}},
  booktitle = {},
  journal = {{A}rchives of {M}icrobiology and {I}mmunology},
  volume = {7},
  numero = {2},
  pages = {65--75},
  ISSN = {2572-9365},
  year = {2023},
  DOI = {10.26502/ami.936500105},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088493},
}