@article{fdi:010088454,
  title = {{F}lying syringes for emerging enzootic virus screening : proof of concept for the development of noninvasive xenosurveillance tools based on {T}setse flies},
  author = {{V}alente, {A}deline and {J}iolle, {D}. and {R}avel, {S}ophie and {P}orciani, {A}ng{\'e}lique and {V}ial, {L}. and {M}ichaud, {V}. and {K}wiatek, {O}. and {P}edarrieu, {A}. and {M}isse, {D}oroth{\'e}e and {F}erraris, {P}auline and {B}retagnolle, {F}. and {B}itome-{E}ssono, {P}.{Y}. and {M}akanga, {B}.{K}. and {R}ougeron, {V}. and {P}rugnolle, {F}. and {P}aupy, {C}hristophe},
  editor = {},
  language = {{ENG}},
  abstract = {{P}athogen transfers between wild and domestic animals and between animals and humans are increasing. {T}heir dramatic consequences for public and veterinary health as well as for conservation call for innovative and user-friendly methods for pathogen surveillance in wildlife.  {X}enosurveillance, a method based on the use of invertebrates (e.g., mosquitoes, hematophagous flies, leeches, cadaveric arthropods) to sample animal tissues (e.g., blood) and the associated pathogens, is one of these tools. {P}reviously, we demonstrated that hematophagous flies, such as tsetse flies, could be useful to detect and identify the etiological agents of malaria in a diverse range of mammals in {G}abon. {H}owever, we did not assess whether this method can be also used to detect viruses. {I}n the present study, we experimentally fed tsetse flies ({G}lossina fuscipes fuscipes) rabbit blood containing different viruses of medical or veterinary importance ({Z}ika, {D}engue, {C}hikungunya, {A}frican swine fever, {B}luetongue, and peste des petits ruminants viruses). {T}hen, we used quantitative {PCR} (i) to determine for how long viral nucleic acid fragments remained detectable in the tsetse midgut during blood digestion and (ii) to compare two blood meal preservation methods (i.e., {FTA} cards and {RNA}later solution) tested using tsetse flies engorged with blood and dengue-2 virus. {A}ll viruses remained detectable for 6 days after feeding, although the detection probability significantly decreased over time. {FTA} cards and {RNA}later solution gave similar results in terms of virus detection. {O}ur results demonstrate that xenosurveillance using blood-engorged tsetse flies is a valuable tool to track and survey viruses in wildlife in {S}ub-{S}aharan {A}frica.},
  keywords = {{AFRIQUE} {SUBSAHARIENNE} ; {GABON}},
  booktitle = {},
  journal = {{T}ransboundary and {E}merging {D}iseases},
  volume = {2023},
  numero = {},
  pages = {145289 [10 ]},
  ISSN = {1865-1674},
  year = {2023},
  DOI = {10.1155/2023/9145289},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088454},
}