@article{fdi:010088367, title = {{A}rthropod-borne virus surveillance as a tool to study the {A}ustralian mosquito virome}, author = {{C}olmant, {A}gathe and {W}arrilow, {D}. and {H}all-{M}endelin, {S}. and {O}nn, {M}. and {H}obson-{P}eters, {J}. and {H}uang, {B}. and {K}urucz, {N}. and {W}archot, {A}. and {P}rimmer, {B}.{R}. and {I}sberg, {S}. and {B}ielefeldt-{O}hmann, {H}. and {H}all, {R}.{A}.}, editor = {}, language = {{ENG}}, abstract = {{M}osquitoes (n = 4381 in 198 pools) were collected in {M}arch and {A}pril 2018 to survey the presence of {W}est {N}ile virus {K}unjin strain in mosquito populations around crocodile farms in the {D}arwin region of the {N}orthern {T}erritory ({NT}) of {A}ustralia. {W}hile no {K}unjin virus was detected in these mosquitoes, we applied our viral replicative intermediates screening system termed monoclonal antibodies to viral {RNA} intermediates in cells or {MAVRIC} to this set of samples. {T}his resulted in the detection of 28 pools with virus replicating in {C}6/36 mosquito cells and the identification of three insect viruses from three distinct virus classes. {W}e demonstrate the persistence of the insect-specific flavivirus {P}alm {C}reek virus in {C}oquillettidia xanthogaster mosquitoes from {D}arwin over almost a decade, with limited genetic drift. {W}e also detected a novel {H}ubei macula-like virus 3 strain in samples from two mosquito genera, suggesting the virus, for which the sequence was originally detected in spiders and soybean thrips, might be involved in a horizontal transmission cycle between arthropods and plants. {O}verall, these data demonstrate the strength of the optimized {MAVRIC} system and contribute to our general knowledge of the mosquito virome and insect viruses.}, keywords = {{AUSTRALIE} ; {DARWIN} ; {TERRITOIRE} {DU} {NORD}}, booktitle = {}, journal = {{V}iruses}, volume = {14}, numero = {9}, pages = {1882 [7 ]}, ISSN = {1999-4915}, year = {2022}, DOI = {10.3390/v14091882}, URL = {https://www.documentation.ird.fr/hor/fdi:010088367}, }