@article{fdi:010088324,
  title = {{D}evelopment of nine microsatellite loci for {T}rypanosoma lewisi, a potential human pathogen in {W}estern {A}frica and {S}outh-{E}ast {A}sia, and preliminary population genetics analyses},
  author = {{S}{\'e}gard, {A}deline and {R}omero, {A}. and {R}avel, {S}ophie and {T}ruc, {P}hilippe and {D}obigny, {G}authier and {G}authier, {P}hilippe and {E}tougbetche, {J}. and {D}ossou, {H}.{J}. and {B}adou, {S}. and {H}ou{\'e}m{\'e}nou, {G}. and {M}orand, {S}. and {C}haisiri, {K}. and {N}oûs, {C}. and {D}e {M}eeûs, {T}hierry},
  editor = {},
  language = {{ENG}},
  abstract = {{T}rypanosoma lewisi belongs to the so-called atypical trypanosomes that occasionally affect humans. {I}t shares the same hosts and flea vector of other medically relevant pathogenic agents as {Y}ersinia pestis, the agent of plague. {I}ncreasing knowledge on the population structure (reproductive mode, population size, dispersal) of this parasite thus represents a challenging but important issue. {T}he use of polymorphic genetic markers, together with suitable population genetics tools, is a convenient way to achieve such objectives. {T}o date, the population biology of {T}. lewisi is poorly known and, to our knowledge, no population genetics studies have ever been conducted. {H}ere, we present the development of nine microsatellite markers of this species. {W}e investigated their polymorphism in different countries from {A}frica and {S}outh-{E}ast {A}sia from {DNA}s extracted from the spleen of their rodent reservoirs (essentially rat species). {S}everal amplification problems arose, especially with {S}outh-{E}ast {A}sian individuals. {T}his led to retain only those individuals with complete genotypes (most of them originating from {W}est {A}frica, notably {C}otonou, {B}enin) to ensure an optimal estimate of heterozygosity. {O}ur results pointed towards a mainly (at least 95-99%) clonal mode of propagation, a strong subdivision at the smallest scale available (i.e., urban neighborhoods, i.e. 0.250 km²), and a generation time most probably shorter than 4 months. {I}n future studies, more extensive sampling at smaller geographic scales (i.e., households), within a one- or two-months window and with improved amplification conditions, should lead to a more precise picture of the fine population structure of this parasite.},
  keywords = {{BENIN} ; {COTONOU} ; {NIGER} ; {NIAMEY}},
  booktitle = {},
  journal = {{P}eer {C}ommunity {J}ournal},
  volume = {2},
  numero = {},
  pages = {e69 [17 ]},
  ISSN = {2804-3871},
  year = {2022},
  DOI = {10.24072/pcjournal.188},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088324},
}