@article{fdi:010088202,
  title = {{W}idespread {B}radyrhizobium distribution of diverse {T}ype {III} effectors that trigger legume nodulation in the absence of {N}od factor},
  author = {{C}amuel, {A}licia and {T}eulet, {A}lbin and {C}arcagno, {M}. and {H}aq, {F}. and {P}acquit, {V}. and {G}ully, {D}jamel and {P}ervent, {M}. and {C}haintreuil, {C}l{\'e}mence and {F}ardoux, {J}o{\¨e}l and {H}orta-{A}raujo, {N}. and {O}kazaki, {S}. and {R}atu, {S}. {T}. {N}. and {G}ueye, {F}. and {Z}illi, {J}. and {N}ouwen, {N}ico and {A}rrighi, {J}ean-{F}ran{\c{c}}ois and {L}uo, {H}. {W}. and {M}ergaert, {P}. and {D}eslandes, {L}. and {G}iraud, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he establishment of the rhizobium-legume symbiosis is generally based on plant perception of {N}od factors ({NF}s) synthesized by the bacteria. {H}owever, some {B}radyrhizobium strains can nodulate certain legume species, such as {A}eschynomene spp. or {G}lycine max, independently of {NF}s, and via two different processes that are distinguished by the necessity or not of a type {III} secretion system ({T}3{SS}). {E}rn{A} is the first known type {III} effector ({T}3{E}) triggering nodulation in {A}eschynomene indica. {I}n this study, a collection of 196 sequenced {B}radyrhizobium strains was tested on {A}. indica. {O}nly strains belonging to the photosynthetic supergroup can develop a {NF}-{T}3{SS}-independent symbiosis, while the ability to use a {T}3{SS}-dependent process is found in multiple supergroups. {O}f these, 14 strains lacking ern{A} were tested by mutagenesis to identify new {T}3{E}s triggering nodulation. {W}e discovered a novel {T}3{E}, {S}up3, a putative {SUMO}-protease without similarity to {E}rn{A}. {I}ts mutation in {B}radyrhizobium strains {NAS}96.2 and {WSM}1744 abolishes nodulation and its introduction in an ern{A} mutant of strain {ORS}3257 restores nodulation. {M}oreover, ectopic expression of sup3 in {A}. indica roots led to the formation of spontaneous nodules. {W}e also report three other new {T}3{E}s, {U}bi1, {U}bi2 and {U}bi3, which each contribute to the nodulation capacity of strain {LMTR}13. {T}hese {T}3{E}s have no homology to known proteins but share with {E}rn{A} three motifs necessary for {E}rn{A} activity. {T}ogether, our results highlight an unsuspected distribution and diversity of {T}3{E}s within the {B}radyrhizobium genus that may contribute to their symbiotic efficiency by participating in triggering legume nodulation.},
  keywords = {},
  booktitle = {},
  journal = {{ISME} {J}ournal},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[14 ]},
  ISSN = {1751-7362},
  year = {2023},
  DOI = {10.1038/s41396-023-01458-1},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088202},
}