Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Bioorganic and Medicinal Chemistry 117366 [17 ] Euphorbiaceae Hura crepitans Daphnane diterpenes Colorectal cancer Caco-2 cells Protein Kinase C 2023 fdi:010088184 ENG Bioorganic and Medicinal Chemistry 0968-0896 ISI:001020510400001 10.1016/j.bmc.2023.117366 https://www.documentation.ird.fr/hor/fdi:010088184 https://www.documentation.ird.fr/intranet/publi/2023-08/010088184.pdf 90 Horizon (IRD) Hura crepitans L. (Euphorbiaceae) is a thorn-covered tree widespread in South America, Africa and Asia which produces an irritating milky latex containing numerous secondary metabolites, notably daphnane-type diter-penes known as Protein Kinase C activators. Fractionation of a dichloromethane extract of the latex led to the isolation of five new daphnane diterpenes (1-5), along with two known analogs (6-7) including huratoxin. Huratoxin (6) and 4 & PRIME;,5 & PRIME;-epoxyhuratoxin (4) were found to exhibit significant and selective cell growth inhibition against colorectal cancer cell line Caco-2 and primary colorectal cancer cells cultured as colonoids. The un-derlying mechanism of 4 and 6 was further investigated revealing the involvement of PKC & zeta;in the cytostatic activity. 076 ; 020 ; 050 UR152 / UR249 Pérou