Daphnanes diterpenes from the latex of Hura crepitans L. and their PKC zeta;-dependent anti-proliferative activity on colorectal cancer cells Crossay, E. /Jullian, Valérie Trinel, M. Sagnat, D. Hamel, D. Groppi, E. Rolland, C. Stigliani, J. L. Mejia, K. Cabanillas, B. J. Alric, L. Buscail, E. El Kalamouni, C. Mavingui, P. Deraison, C. Racaud-Sultan, C. Fabre, N. Euphorbiaceae Hura crepitans Daphnane diterpenes Colorectal cancer Caco-2 cells Protein Kinase C Hura crepitans L. (Euphorbiaceae) is a thorn-covered tree widespread in South America, Africa and Asia which produces an irritating milky latex containing numerous secondary metabolites, notably daphnane-type diter-penes known as Protein Kinase C activators. Fractionation of a dichloromethane extract of the latex led to the isolation of five new daphnane diterpenes (1-5), along with two known analogs (6-7) including huratoxin. Huratoxin (6) and 4 & PRIME;,5 & PRIME;-epoxyhuratoxin (4) were found to exhibit significant and selective cell growth inhibition against colorectal cancer cell line Caco-2 and primary colorectal cancer cells cultured as colonoids. The un-derlying mechanism of 4 and 6 was further investigated revealing the involvement of PKC & zeta;in the cytostatic activity. 2023 text https://www.documentation.ird.fr/hor/fdi:010088184 fdi:010088184 Crossay E., Jullian Valérie, Trinel M., Sagnat D., Hamel D., Groppi E., Rolland C., Stigliani J. L., Mejia K., Cabanillas B. J., Alric L., Buscail E., El Kalamouni C., Mavingui P., Deraison C., Racaud-Sultan C., Fabre N.. Daphnanes diterpenes from the latex of Hura crepitans L. and their PKC zeta;-dependent anti-proliferative activity on colorectal cancer cells. 2023, 90, 117366 [17 ] EN