@article{fdi:010088126,
  title = {{L}arge-scale analysis of putative plasmids in clinical multidrug-resistant {E}scherichia coli isolates from {V}ietnamese patients},
  author = {{N}guyen, {Q}. {H}. and {L}e, {T}. {T}. {H}. and {N}guyen, {S}. {T}. and {N}guyen, {K}. {O}. {T}. and {Q}uyen, {D}. {V}. and {H}ayer, {J}uliette and {B}anuls, {A}nne-{L}aure and {T}ran, {T}. {T}. {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}ntroduction{I}n the past decades, extended-spectrum beta-lactamase ({ESBL})-producing and carbapenem-resistant ({CR}) {E}scherichia coli isolates have been detected in {V}ietnamese hospitals. {T}he transfer of antimicrobial resistance ({AMR}) genes carried on plasmids is mainly responsible for the emergence of multidrug-resistant {E}. coli strains and the spread of {AMR} genes through horizontal gene transfer. {T}herefore, it is important to thoroughly study the characteristics of {AMR} gene-harboring plasmids in clinical multidrug-resistant bacterial isolates. {M}ethods{T}he profiles of plasmid assemblies were determined by analyzing previously published whole-genome sequencing data of 751 multidrug-resistant {E}. coli isolates from {V}ietnamese hospitals in order to identify the risk of {AMR} gene horizontal transfer and dissemination. {R}esults{T}he number of putative plasmids in isolates was independent of the sequencing coverage. {T}hese putative plasmids originated from various bacterial species, but mostly from the {E}scherichia genus, particularly {E}. coli species. {M}any different {AMR} genes were detected in plasmid contigs of the studied isolates, and their number was higher in {CR} isolates than in {ESBL}-producing isolates. {S}imilarly, the bla({KPC}-2), bla({NDM}-5), bla({OXA}-1), bla({OXA}-48), and bla({OXA}-181) beta-lactamase genes, associated with resistance to carbapenems, were more frequent in {CR} strains. {S}equence similarity network and genome annotation analyses revealed high conservation of the beta-lactamase gene clusters in plasmid contigs that carried the same {AMR} genes. {D}iscussion{O}ur study provides evidence of horizontal gene transfer in multidrug-resistant {E}. coli isolates via conjugative plasmids, thus rapidly accelerating the emergence of resistant bacteria. {B}esides reducing antibiotic misuse, prevention of plasmid transmission also is essential to limit antibiotic resistance.},
  keywords = {{E}scherichia coli ; putative plasmid ; carbapenem resistance ; horizontal ; gene transfer ; whole-genome sequencing ; {VIET} {NAM}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {14},
  numero = {},
  pages = {1094119 [16 ]},
  year = {2023},
  DOI = {10.3389/fmicb.2023.1094119},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088126},
}