@article{fdi:010088051,
  title = {3{D} cellular morphometrics of ovule primordium development in {Z}ea mays reveal differential division and growth dynamics specifying megaspore mother cell singleness},
  author = {{O}uedraogo, {I}nes and {L}artaud, {M}. and {B}aroux, {C}. and {M}osca, {G}. and {D}elgado, {L}. and {L}eblanc, {O}. and {V}erdeil, {J}. {L}. and {C}onejero, {G}. and {A}utran, {D}aphn{\'e}},
  editor = {},
  language = {{ENG}},
  abstract = {{I}ntroduction{D}ifferentiation of spore mother cells marks the somatic-to-reproductive transition in higher plants. {S}pore mother cells are critical for fitness because they differentiate into gametes, leading to fertilization and seed formation. {T}he female spore mother cell is called the megaspore mother cell ({MMC}) and is specified in the ovule primordium. {T}he number of {MMC}s varies by species and genetic background, but in most cases, only a single mature {MMC} enters meiosis to form the embryo sac. {M}ultiple candidate {MMC} precursor cells have been identified in both rice and {A}rabidopsis, so variability in {MMC} number is likely due to conserved early morphogenetic events. {I}n {A}rabidopsis, the restriction of a single {MMC} per ovule, or {MMC} singleness, is determined by ovule geometry. {T}o look for potential conservation of {MMC} ontogeny and specification mechanisms, we undertook a morphogenetic description of ovule primordium growth at cellular resolution in the model crop maize. {M}ethods{W}e generated a collection of 48 three-dimensional (3{D}) ovule primordium images for five developmental stages, annotated for 11 cell types. {Q}uantitative analysis of ovule and cell morphological descriptors allowed the reconstruction of a plausible developmental trajectory of the {MMC} and its neighbors. {R}esults{T}he {MMC} is specified within a niche of enlarged, homogenous {L}2 cells, forming a pool of candidate archesporial ({MMC} progenitor) cells. {A} prevalent periclinal division of the uppermost central archesporial cell formed the apical {MMC} and the underlying cell, a presumptive stack cell. {T}he {MMC} stopped dividing and expanded, acquiring an anisotropic, trapezoidal shape. {B}y contrast, periclinal divisions continued in {L}2 neighbor cells, resulting in a single central {MMC}. {D}iscussion{W}e propose a model where anisotropic ovule growth in maize drives {L}2 divisions and {MMC} elongation, coupling ovule geometry with {MMC} fate.},
  keywords = {ovule primordium ; 3{D} morphometrics ; developmental atlas ; morphogenesis ; {Z}ea mays ; female germ cell ; {MMC} specification},
  booktitle = {},
  journal = {{F}rontiers in {P}lant {S}cience},
  volume = {14},
  numero = {},
  pages = {1174171 [17 p.]},
  ISSN = {1664-462{X}},
  year = {2023},
  DOI = {10.3389/fpls.2023.1174171},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088051},
}