Ouass S. auteur aut IRD Boulanger N. auteur aut IRD Lelouvier B. auteur aut IRD Insonere J. L. M. auteur aut IRD Lacroux C. auteur aut IRD Krief S. auteur aut IRD Asalu E. auteur aut IRD Rahola Nil auteur aut IRD Duron O. auteur aut IRD text journalArticle eng text/pdf born digital access The family Anaplasmataceae includes tick-borne bacteria of major public and veterinary health interest, as best illustrated by members of the genera Anaplasma and Ehrlichia. Recent epidemiological surveys have also reported on the presence of a novel putative genus in the Anaplasmataceae, Candidatus Allocryptoplasma, previously described as Candidatus Cryptoplasma in the western black-legged tick, Ixodes pacificus. However, the genetic diversity of Ca. Allocryptoplasma and its phylogenetic relationship with other Anaplasmataceae remain unclear. In this study, we developed a multi-locus sequence typing approach, examining the DNA sequence variation at five genes of Ca. Allocryptoplasma found in ticks. Combining this multi-locus sequence typing and genetic data available on public databases, we found that substantial genetic diversity of Ca. Allocryptoplasma is present in Ixodes, Amblyomma and Haemaphysalis spp. ticks on most continents. Further analyses confirmed that the Ca. Allocryptoplasma of ticks, the Ca. Allocryptoplasma of lizards and some Anaplasma-like bacteria of wild mice cluster into a monophyletic genus, divergent from all other genera of the family Anaplasmataceae. Candidatus Allocryptoplasma appears as a sister genus of Anaplasma and, with the genera Ehrlichia and Neoehrlichia, they form a monophyletic subgroup of Anaplasmataceae associated with tick-borne diseases. The detection of genetically distinct Ca. Allocryptoplasma in ticks of significant medical or veterinary interest supports the hypothesis that it is an emergent genus of tick-borne pathogens of general concern. specialized Anaplasmataceae Anaplasma Ehrlichia Allocryptoplasma Tick Tick-borne diseases 052 30 13 [9 ] 2023 1252-607X https://www.documentation.ird.fr/hor/fdi:010087773 10.1051/parasite/2023014 1252-607X [F B010087773] https://www.documentation.ird.fr/hor/fdi:010087773 https://www.documentation.ird.fr/intranet/publi/2023-07/010087773.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2023-07-06 2025-02-24 fdi:010087773 fre