@article{fdi:010087747,
  title = {{M}olecular tools to infer resistance-breaking abilities of {R}ice {Y}ellow {M}ottle {V}irus isolates},
  author = {{D}ossou, {L}. and {G}alzi, {A}gn{\`e}s and {A}ribi, {J}amel and {P}oulicard, {N}ils and {A}lbar, {L}aurence and {F}atogoma, {S}. and {N}djiondjop, {M}. {N}. and {K}one, {D}. and {H}{\'e}brard, {E}ug{\'e}nie},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice yellow mottle virus ({RYMV}) is a major biotic constraint to rice cultivation in {A}frica. {RYMV} shows a high genetic diversity. {V}iral lineages were defined according to the coat protein ({CP}) phylogeny. {V}arietal selection is considered as the most efficient way to manage {RYMV}. {S}ources of high resistance were identified mostly in accessions of the {A}frican rice species, {O}ryza glaberrima. {E}mergence of resistance-breaking ({RB}) genotypes was observed in controlled conditions. {T}he {RB} ability was highly contrasted, depending on the resistance sources and on the {RYMV} lineages. {A} molecular marker linked to the adaptation to susceptible and resistant {O}. glaberrima was identified in the viral protein genome-linked ({VP}g). {B}y contrast, as no molecular method was available to identify the hypervirulent lineage able to overcome all known resistance sources, plant inoculation assays were still required. {H}ere, we designed specific {RT}-{PCR} primers to infer the {RB} abilities of {RYMV} isolates without greenhouse experiments or sequencing steps. {T}hese primers were tested and validated on 52 isolates, representative of {RYMV} genetic diversity. {T}he molecular tools described in this study will contribute to optimizing the deployment strategy of resistant lines, considering the {RYMV} lineages identified in fields and their potential adaptability.},
  keywords = {{RYMV} ; rice ; resistance ; virulence ; detection ; {AFRIQUE}},
  booktitle = {},
  journal = {{V}iruses},
  volume = {15},
  numero = {4},
  pages = {959 [12 p.]},
  year = {2023},
  DOI = {10.3390/v15040959},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087747},
}