@article{fdi:010087701,
  title = {{A}re international units of anti-{HB}s antibodies always indicative of hepatitis {B} virus neutralizing activity ?},
  author = {{A}ronthippaitoon, {Y}. and {S}zerman, {N}. and {N}go-{G}iang-{H}uong, {N}icole and {L}aperche, {S}. and {U}ngeheuer, {M}. {N}. and {S}ureau, {C}. and {K}hamduang, {W}. and {G}audy-{G}raffin, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective: {A}nti-{HB}s antibodies are elicited upon hepatitis {B} vaccination, and concentrations above 10 m{IU}/m{L} are considered protective. {O}ur aim was to assess the relationship between {IU}/m{L} of anti-{HB}s and neutralization activity. {M}ethods: {I}mmunoglobulins {G} ({I}g{G}s) were purified from individuals who received a serum-derived vaccine ({G}roup 1), a recombinant vaccine, {G}enevac-{B} or {E}ngerix-{B} ({G}roup 2), or who recovered from acute infection ({G}roup 3). {I}g{G}s were tested for anti-{HB}s, anti-pre{S}1, and anti-pre{S}2 antibodies and for their neutralizing activity in an in vitro infection assay. {R}esults: {A}nti-{HB}s {IU}s/m{L} value did not strictly correlate with neutralization activity. {T}he {G}roup 1 antibodies demonstrated a greater neutralizing activity than those of {G}roup 2. {A}nti-pre{S}1 antibodies were detected in {G}roups 1 and 3, and anti-pre{S}2 in {G}roup 1 and {G}roup 2/{G}enhevac-{B}, but the contribution of anti-pre{S} antibodies to neutralization could not be demonstrated. {V}irions bearing immune escape {HB}s{A}g variants were less susceptible to neutralization than wild-type virions. {C}onclusion. {T}he level of anti-{HB}s antibodies in {IU}s is not sufficient to assess neutralizing activity. {C}onsequently, (i) an in vitro neutralization assay should be included in the quality control procedures of antibody preparations intended for {HB} prophylaxis or immunotherapy, and (ii) a greater emphasis should be placed on ensuring that vaccine genotype/subtype matches with that of the circulating {HBV}.},
  keywords = {{HBV} vaccine ; anti-{HB}s international units ; anti-{HB}s neutralizing activity ; {HBV} infectivity neutralization},
  booktitle = {},
  journal = {{V}accines},
  volume = {11},
  numero = {4},
  pages = {791 [14 p.]},
  year = {2023},
  DOI = {10.3390/vaccines11040791},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087701},
}