@article{fdi:010087615,
  title = {{D}iversity of microbiomes across a 13,000-year-old {A}mazon sediment},
  author = {{T}hompson, {C}. {C}. and {T}schoeke, {D}. and {C}outinho, {F}. {H}. and {L}eomil, {L}. and {G}arcia, {G}. {D}. and {O}tsuki, {K}. and {T}urcq, {B}runo and {M}oreira, {L}. {S}. and {M}oreira {T}urcq, {P}atricia and {C}ordeiro, {R}. {C}. and {A}sp, {N}. {E}. and {T}hompson, {F}. {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he microbiome is fundamental for understanding bacterial activities in sediments. {H}owever, only a limited number of studies have addressed the microbial diversity of {A}mazonian sediments. {H}ere, we studied the microbiome of sediments from a 13,000-year {BP} core retrieved in a floodplain lake in {A}mazonia using metagenomics and biogeochemistry. {O}ur aim was to evaluate the possible environmental influence over a river to a lake transition using a core sample. {T}o this end, we sampled a core in the {A}iro {L}ake, a floodplain lake in the {N}egro {R}iver basin. {T}he {N}egro {R}iver is the largest tributary of the {A}mazon {R}iver. {T}he obtained core was divided into three strata: (i) surface, almost complete separation of the {A}iro {L}ake from the {N}egro {R}iver when the environment becomes more lentic with greater deposition of organic matter (black-colored sediment); (ii) transitional environment (reddish brown); and (iii) deep, environment with a tendency for greater past influence of the {N}egro {R}iver (brown color). {T}he deepest sample possibly had the greatest influence of the {N}egro {R}iver as it represented the bottom of this river in the past, while the surface sample is the current {A}iro {L}ake bottom. {I}n total, six metagenomes were obtained from the three different depth strata (total number of reads: 10.560.701; sequence length: 538 +/- 24, mean +/- standard deviation). {T}he older (deeper) sediment strata contained a higher abundance of {B}urkholderia, {C}hitinophaga, {M}ucilaginibacter, and {G}eobacter, which represented similar to 25% of the metagenomic sequences. {O}n the other hand, the more recent sediment strata had mainly {T}hermococcus, {T}ermophilum, {S}ulfolobus, {A}rchaeoglobus, and {M}ethanosarcina (in total 11% of the metagenomic sequences). {T}he sequence data were binned into metagenome-assembled genomes ({MAG}s). {T}he majority of the obtained {MAG}s (n = 16) corresponded to unknown taxa, suggesting they may belong to new species. {T}he older strata sediment microbiome was enriched with sulfur cycle genes, {TCA} cycle, {Y}gf{Z}, and {ATP}-dependent proteolysis in bacteria. {M}eanwhile, serine-glyoxylate cycle, stress response genes, bacterial cell division, cell division-ribosomal stress protein cluster, and oxidative stress increased in the younger strata. {M}etal resistance and antimicrobial resistance genes were found across the entire core, including genes coding for fluoroquinolones, polymyxin, vancomycin, and multidrug resistance transporters. {T}hese findings depict the possible microbial diversity during the depositional past events and provided clues of the past microbial metabolism throughout time.},
  keywords = {{M}icrobiome ; 13000-year-old {A}mazon sediment ; {A}ntibiotic resistance genes ; {AMAZONIE}},
  booktitle = {},
  journal = {{M}icrobial {E}cology},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[8 ]},
  ISSN = {0095-3628},
  year = {2023},
  DOI = {10.1007/s00248-023-02202-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087615},
}