@article{fdi:010087613,
  title = {{M}osquito bites and stage-specific antibody responses against {P}lasmodium falciparum in southern {G}hana},
  author = {{K}wapong, {S}. {S}. and {A}sare, {K}. {K}. and {K}usi, {K}. {A}. and {P}appoe, {F}. and {N}dam, {N}. and {T}ahar, {R}achida and {P}oinsignon, {A}nne and {A}moah, {L}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground{T}he human host elicits specific immune responses after exposure to various life stages of the malaria parasite as well as components of mosquito saliva injected into the host during a mosquito bite. {T}his study describes differences in {I}g{G} responses against antigens derived from the sporozoite ({P}f{CSP}), asexual stage parasite ({P}f{EBA}175) and the gametocyte ({P}fs230), in addition to an {A}nopheles gambiae salivary gland antigen (g{SG}6-{P}1), in two communities in {G}hana with similar blood stage malaria parasite prevalence.{M}ethods{T}his study used archived plasma samples collected from an earlier cross-sectional study that enrolled volunteers aged from 6 months to 70 years from {S}imiw, peri-urban community ({N} = 347) and {O}bom, rural community ({N} = 291). {A}n archived thick and thin blood smear for microscopy was used for the estimation of {P}lasmodium parasite density and species and {DNA} extraction from blood spots and {P}. falciparum confirmation was performed using {PCR}. {T}his study used the stored plasma samples to determine {I}g{G} antibody levels to {P}. falciparum and {A}nopheles salivary antigens using indirect {ELISA}.{R}esults{I}ndividuals from {S}imiw had significantly higher levels of {I}g{G} against mosquito g{SG}6-{P}1 [median (95%{CI})] [2.590 (2.452-2.783) ng/m{L}] compared to those from {O}bom [2.119 (1.957-2.345) ng/m{L}], p < 0.0001. {B}oth {I}g{G} responses against {P}fs230pro{C} (p = 0.0006), and {P}f{CSP} (p = 0.002) were significantly lower in volunteers from {S}imiw compared to the participants from {O}bom. {T}he seroprevalence of {P}f{EBA}-175.5{R} (p = 0.8613), g{SG}6-{P}1 (p = 0.0704), {P}f{CSP} (p = 0.7798) {I}g{G} were all similar in {O}bom and {S}imiw. {H}owever, {P}fs230 seroprevalence was significantly higher at {O}bom compared to {S}imiw (p = 0.0006). {S}pearman correlation analysis showed no significant association between {I}g{G} responses against g{SG}6-{P}1, {P}f{CSP}, {P}fs230pro{C} and {P}f{EBA}-175.5{R} and parasite density at both {O}bom and {S}imiw (p > 0.05).{C}onclusion{I}n conclusion, the study showed that participants from {S}imiw had higher concentrations of circulating g{SG}6-{P}1 {I}g{G} antibodies but lower concentrations of {P}. falciparum antibodies, {P}f{CSP} {I}g{G} and {P}fs230pro{C} {I}g{G} compared to participants from {O}bom.},
  keywords = {{P}lasmodium falciparum ; {C}ircumsporozoite surface protein ({P}f{CSP}) ; {E}rythrocyte binding antigen 175 ({P}f{EBA}175) ; {A}nopheles gambiae salivary ; protein g{SG}6 peptide 1 (g{SG}6-{P}1) ; {M}alaria transmission ; {G}hana ; {GHANA}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {22},
  numero = {1},
  pages = {126 [12 p.]},
  year = {2023},
  DOI = {10.1186/s12936-023-04557-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087613},
}