@article{fdi:010087568, title = {{A}rtificial substrates coupled with q{PCR} ({AS}-q{PCR}) assay for the detection of the toxic benthopelagic dinoflagellate {V}ulcanodinium rugosum}, author = {{B}ouquet, {A}. and {F}elix, {C}. and {M}asseret, {E}. and {R}eymond, {C}. and {A}badie, {E}. and {L}aabir, {M}ohamed and {R}olland, {J}. {L}.}, editor = {}, language = {{ENG}}, abstract = {{V}ulcanodinium rugosum is an emerging benthopelagic neuro-toxic dinoflagellate species responsible for seasonal {P}innatoxins and {P}ortimines contaminations of shellfish and marine animals. {T}his species is challenging to detect in the environment, as it is present in low abundance and difficult to be identified using light microscopy. {I}n this work, we developed a method using artificial substrates coupled with q{PCR} ({AS}-q{PCR}) to detect {V}. rugosum in a marine environment. {T}his sensitive, specific and easy-to-standardize alternative to current techniques does not require specialized expertise in taxonomy. {A}fter determining the limits and specificity of the q{PCR}, we searched for the presence of {V}. rugosum in four {F}rench {M}editerranean lagoons using artificial substrates collected every two weeks for one year. {T}he {AS}-q{PCR} method revealed its occurrences in summer 2021 in every studied lagoon and detected cells in more samples than light microscopy. {A}s {V}. rugosum development induces shellfish contamination even at low microalga densities, the {AS}-q{PCR} method is accurate and relevant for monitoring {V}. rugosum in a marine environment.}, keywords = {artificial substrate ; {PCR} ; detection ; {V}ulcanodinium rugosum ; toxins ; benthopelagic ; {FRANCE} ; {ZONE} {MEDITERRANEENNE}}, booktitle = {}, journal = {{T}oxins}, volume = {15}, numero = {3}, pages = {217 [11 ]}, year = {2023}, DOI = {10.3390/toxins15030217}, URL = {https://www.documentation.ird.fr/hor/fdi:010087568}, }