Fenouil R. auteur aut IRD Pradel Nathalie auteur aut IRD Belahbib H. auteur aut IRD Roumagnac M. auteur aut IRD Bartoli M. auteur aut IRD Ben Hania W. auteur aut IRD Denis Y. auteur aut IRD Garel M. auteur aut IRD Tamburini C. auteur aut IRD Ollivier Bernard auteur aut IRD Summers Z. auteur aut IRD Armougom Fabrice auteur aut IRD Dolla A. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Pseudothermotoga elfii strain DSM9442 and P. elfii subsp. lettingae strain DSM14385 are hyperthermophilic bacteria. P. elfii DSM9442 is a piezophile and was isolated from a depth of over 1600 m in an oil-producing well in Africa. P. elfii subsp. lettingae is piezotolerant and was isolated from a thermophilic bioreactor fed with methanol as the sole carbon and energy source. In this study, we analyzed both strains at the genomic and transcriptomic levels, paying particular attention to changes in response to pressure increases. Transcriptomic analyses revealed common traits of adaptation to increasing hydrostatic pressure in both strains, namely, variations in transport membrane or carbohydrate metabolism, as well as species-specific adaptations such as variations in amino acid metabolism and transport for the deep P. elfii DSM9442 strain. Notably, this work highlights the central role played by the amino acid aspartate as a key intermediate of the pressure adaptation mechanisms in the deep strain P. elfii DSM9442. Our comparative genomic and transcriptomic analysis revealed a gene cluster involved in lipid metabolism that is specific to the deep strain and that was differentially expressed at high hydrostatic pressures and might, thus, be a good candidate for a piezophilic gene marker in Pseudothermotogales. specialized hydrostatic pressure adaptation Pseudothermotoga sp 084 020 11 3 773 [17 ] 2023 https://www.documentation.ird.fr/hor/fdi:010087565 10.3390/microorganisms11030773 [F B010087565] https://www.documentation.ird.fr/hor/fdi:010087565 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-05/010087565.pdf IRD - Base Horizon / Pleins textes 2023-05-12 2025-02-24 fdi:010087565 fre